摘要
建立一种测定食品中甜蜜素含量的高效液相色谱法。采用超纯水对样品进行提取,甜蜜素衍生化反应后通过高效液相色谱仪进行定性定量,讨论前处理对于甜蜜素回收率的影响。结果表明甜蜜素浓度在0~100μg/mL范围内,线性关系良好(R^2﹥0.9998),方法的最低检出限为0.42μg/mL,回收率为90.2%~99.3%,相对标准偏差(RSD)为1.1%~2.5%。该方法操作简便、回收率高、检测限低,可用于食品中甜蜜素含量的测定。
A method for determination of sodium cyclamate in food was established by high performance liquid chromatography. The samples were extracted with ultra-pure water, and the qualitative and quantitative analysis of sodium cyclamate derivatized reactants in food was analyzed by high performance liquid chromatography, and the effect of pre-processing of sodium cyclamate recovery in fond was discussed. Results Sodium cyclamate had a good linear relationships in the range of 0~100 μg/mL(R^2﹥0.9998) with the limits of detection was 0.42 mg/kg. The recovery was 90.2 %~99.3 % and the relative standard deviation(RSD) was 1.1 %~2.5 %. This method has great operability, high sensitivity, high recovery, low limits of detection, which is suitable for the determination of sodium cyclamate in food.
作者
陈营寿
李康艳
唐成彦
符映英
曾丹丹
Chen Yingshou;Li Kangyan;Tang Chengyan;Fu Yingying;Zeng Dandan(Zhanjiang Institute for Food and Drug Control,Zhanjiang 524100;Lingnan Normal University,Zhanjiang 524048,China)
出处
《广东化工》
CAS
2020年第4期166-168,共3页
Guangdong Chemical Industry
基金
湛江市科技项目《食品中甜味剂的现场快速检测技术研究》(项目编号为2015A03033)。
关键词
高效液相色谱法
甜蜜素
添加剂
high performance liquid chromatography
sodium cyclamate
additive
