摘要
目的分析河南省早产儿、足月儿和过期产儿干血斑中游离肉碱和酰基肉碱水平并分别建立其参考区间。方法选取2015年1月至2019年6月于河南省出生的63799例新生儿作为研究对象,根据出生时胎龄分为早产儿组(n=10034)、足月儿组(n=51307)和过期产儿组(n=2458),采用串联质谱法对其游离肉碱和酰基肉碱进行检测及比较,根据出生时胎龄分别建立其参考区间。结果足月儿与早产儿的游离肉碱和除C4外的酰基肉碱水平的差异均具有统计学意义(P<0.05);足月儿的酰基肉碱水平显著高于过期产儿(P<0.05);早产儿与过期产儿的游离肉碱和除C2、C3 DC/C4 OH、C6、C10、C14和C18外的酰基肉碱水平的差异亦具有统计学意义(P<0.05);按照不同胎龄新生儿分别建立了其游离肉碱和酰基肉碱的参考区间。结论分别建立了河南省早产儿、过期产儿和足月儿游离肉碱和酰基肉碱的参考区间,为河南省新生儿脂肪酸和有机酸代谢障碍性疾病的筛查及诊断提供了参考依据。
Objective To determine levels for the free carnitine and acylcarnitines in dry blood spots of preterm,full term and postterm infants from Henan province,and to establish their reference intervals respectively.Methods A total of 63799 newborns born in Henan Province from January,2015 to June,2019 were enrolled as the study subjects.According to the gestational age at birth,they were divided into the preterm infant group(n=10034),the full term infant group(n=51307)and the postterm infant group(n=2458).The levels of free carnitine and acylcarnitine were tested by Tandem mass spectrometry,and the reference intervals were established on the basis of gestational age at birth.Results The levels of free carnitine and acylcarnitines except for C4 had statistical differences between full term and preterm infants(P<0.05).The levels of acylcarnitine in full term infants were significantly higher than those in postterm infants(P<0.05).Except for C2,C3DC/C4OH,C6,C10,C14 and C18,the levels of free carnitine and acylcarnitine were significantly different between preterm and postterm infants(P<0.05).The reference intervals of newborns were established according to different gestational age.Conclusion The reference intervals of free carnitine and acylcarnitine for preterm infants,postterm infants and full-term infants in Henan Province are established respectively,which can be used as the reference basis for screening and diagnosing disorders of fatty acid and organic acid metabolism of newborns in Henan province.
作者
贾晨路
苏立
任益慧
李艳茹
李润卿
赵德华
JIA Chenlu;SU li;REN Yihui;LI Yanru;LI Runqing;ZHAO Dehua(The Neonatal Screening Center of Henan Province,The Third Affiliated Hospital of Zhengzhou University,Zhengzhou 450052, China)
出处
《标记免疫分析与临床》
CAS
2020年第3期365-368,共4页
Labeled Immunoassays and Clinical Medicine
基金
河南省科技攻关计划项目(编号:172102310646)
河南省医学教育研究项目(编号:wjlx2019054)。
关键词
新生儿
早产儿
过期产儿
游离肉碱
酰基肉碱
参考区间
Newborn
Preterm infant
Postterm infant
Free carnitine
Acylcarnitine
Reference interval
