摘要
目的分析miR-218-5p在胃癌细胞和胃癌组织中的表达,采用生物信息学方法,预测其靶基因并对其信号通路进行富集分析,并利用OncomiR数据库对miR-218-5p在胃癌中的表达与预后关系进行生存分析。方法通过实时荧光定量PCR检测miR-218-5p在不同分化程度的胃癌细胞和正常细胞、胃癌组织和癌旁组织中的表达,分析其临床意义。在miRecords网站数据库选择3个以上软件在线预测其靶基因,取其交集,运用DAVID6.7数据库,对其靶基因参与的信号通路进行富集分析。采用OncomiR数据库分析miR-218-5p在胃癌组织相对于癌旁组织中的表达,并利用OncomiR和cBioportal数据库分别进行miR-218-5p在胃癌组织中的表达与预后生存期的分析。结果miR-218-5p在不同分化程度的胃癌细胞中表达下调。相对于癌旁组织,miR-218-5p在胃癌组织中也明显下调,miR-218-5p的下调与胃癌淋巴结转移(P<0.001)及TNM分期(P<0.001)具有相关性。生物信息学分析显示,miR-218-5p的靶基因富集在与肿瘤相关的多个信号通路中,且其在胃癌中的表达与胃癌患者预后具有一定相关性(P=0.01632)。结论miR-218-5p在胃癌中表达下调,起到潜在的抑癌作用,可能成为新的治疗靶点和预后标志物。
Objective To analyze the expression of miR-218-5p in gastric cancer cells and gastric cancer tissues,and to predict its target genes and enrich the signal pathways by bioinformatics method,and to analyze the relationship between expression of miR-218-5p and prognosis in gastric cancer using OncomiR database.Methods Real-time fluorescence-determined PCR was used to detect the expression of miR-218-5p in gastric cancer cells and normal gastric cancer cells with different degrees of differentiation,as well as the expression levels in gastric cancer tissues and adjacent tissues.Its clinical significance was analyze.Three or more software were selected in the miRecords website database to predict their target genes online,and the intersections were taken.The DAVID6.7 database was used to enrich the signal pathways involved in the target genes.Expression of miR-218-5p in gastric cancer tissues was compared with normal tissues by OncomiR database,and expression of miR-218-5p in gastric cancer tissues and prognosis survival were analyzed by OncomiR and cBioportal databases,respectively.Results The expression of miR-218-5p was down-regulated in gastric cancer cells with different degrees of differentiation.Compared with precancerous tissues,miR-218-5p was down-regulated in gastric cancer tissues.The down-regulated expression of miR-218-5p was associated with lymph node metastasis(P<0.001)and TNM stage(P<0.001)of gastric cancer.Informatics analysis revealed that the target gene of miR-218-5p was enriched in multiple signaling pathways associated with tumors,and its expression in gastric cancer was significantly correlated with the prognosis of patients with gastric cancer(P=0.01632).Conclusion The expression of miR-218-5p is down-regulated in gastric cancer,which has potential anti-cancer effects and may become a new therapeutic target and prognostic marker.
作者
杜正平
李海龙
刘春萍
王转兄
张雪燕
顾静
徐燕
DU Zhengping;LI Hailong;LIU Chunping;WANG Zhuanxiong;ZHANG Xueyan;GU Jing;XU yan(Clinical Medical College of Gansu University of Chinese Medicine,Lanzhou,Gansu 730030,China;Gansu Provincial Key Laboratory of Traditional Chinese Medicine Exploitation and Innovation,Lanzhou,Gansu 730030,China;Department of Clinical Laboratory,Second People′s Hospital of Jiuquan,Jiuquan,Gansu 735000,China;Laboratory Medicine,Gansu Healthy Vocational College,Lanzhou,Gansu 730030,China;Department of Physiology,Basic Medical College of Gansu University of Chinese Medicine,Lanzhou,Gansu 730030,China;Department of Editorial,Journal of Gansu University of Chinese Medicine,Lanzhou,Gansu 730030,China)
出处
《检验医学与临床》
CAS
2020年第7期876-880,共5页
Laboratory Medicine and Clinic
基金
甘肃省自然科学基金项目(17JR5RA173)
甘肃省高等学校科研项目(甘财教[2014]63-15)
甘肃省中医药大学研究生创新基金(CX2019-26)。
