摘要
为探究青海野生草地早熟禾(Poa pratensis)低温胁迫下的分子应答机制,本试验以青海省野生草地早熟禾耐寒材料‘10-122’和低温敏感材料‘09-126’为研究对象,采用高通量Illumina Hiseq测序技术分析了在低温胁迫与常温对照间的转录组差异。结果表明:‘10-122’共有31943个差异表达基因,其中,17088个差异表达基因上调表达,14855个差异表达基因下调表达;‘09-126’共有25905个差异表达基因,其中,13513个差异表达基因上调表达,12392个差异表达基因下调表达;对2种材料进行差异表达基因富集分析,得出差异表达基因低温胁迫下在光合作用、氧化还原反应过程、碳水化合物代谢、细胞膜系统、转运蛋白以及次生物代谢中富集显著;此外,一些钙信号调节、激素代谢和信号传导、抗氧化系统、碳水化合物代谢等通路的基因仅在耐寒型种质材料‘10-122’上调表达,可作为潜在的抗寒基因,如CML、CPK、CALM、DHAR、GST、NCED、SNRK2、BSK、CKX、BIN2、ARF和PEK等。
In order to explore the molecular response mechanism to low temperature stress of Qinghai wild Poa pratensis,this experiment took cold-resistant germplasm material‘10-122’and low-temperature sensitive germplasm material‘09-126’as research objects and analyzed transcriptome differences between low temperature stress and normal temperature controls with high-throughput Illumina Hiseq sequencing technology.The results showed that there were 31943 differentially expressed genes detected in‘10-122’,in which 17088 differentially expressed genes were up-regulated and 14855 differentially expressed genes were down-regulated.There were 25905 differentially expressed genes detected in‘09-126’,in which 13513 differentially expressed genes were up-regulated and 12392 differentially expressed genes were down-regulated.The enrichment analysis of differentially expressed genes on two germplasm materials showed that the differentially expressed genes were significantly enriched in photosynthesis,oxidation-reduction,carbohydrate metabolism,cell membrane system,transporter protein,and secondary metabolism under low temperature stress.Additionally,a number of DEGs involved in Ca 2+signaling,plant hormone biosynthesis and signaling transduction,antioxidant system and carbohydrate metabolism pathways were specific up-regulated in cold-resistant genotype‘10-122’.Therefore,these DEGs including CML、CPK、CALM、DHAR、GST、NCED、SNRK2、BSK、CKX、BIN2、ARF were identified as potential genes associated with the cold resistance of‘10-122’genotype.
作者
赵春旭
马祥
董文科
牛奎举
张然
马晖玲
ZHAO Chun-xu;MA Xiang;Dong Wen-ke;Niu Kui-ju;Zhang Ran;MA Hui-ling(College of Pratacultural Science,Gansu Agricultural University,Key Laboratory of Grassland Ecosystem,Ministry of Education,Pratacultural Engineering Laboratory of Gansu Province,Sino-U.S.Center for Grassland Ecosystem Sustainability,Lanzhou,Gansu Province 730070,China;Key Laboratory of Superior Forage Germplasm in the Qinghai-Tibetan Plateau,Qinghai Academy of Animal Science and Veterinary Medicine,Qinghai University,Xining,Qinghai Province 810016,China)
出处
《草地学报》
CAS
CSCD
北大核心
2020年第2期305-318,共14页
Acta Agrestia Sinica
基金
青海省科技厅重点实验室发展专项“青海省青藏高原优良牧草种质资源利用重点实验室”(2020-ZJ-Y03)资助。
关键词
低温胁迫
野生草地早熟禾
转录组
差异表达基因
Low-temperature stress
wild Poa pratensis
Transcriptome
Differentially expressed genes
