摘要
目的寻求“见肝之病,知肝传脾,当先实脾”的生物学依据;探讨其对肝郁叠加慢性肝损伤大鼠模型肝细胞凋亡的影响。方法将48只大鼠采用随机数字表法分为6组,分别为正常组、模型组、双环醇组、柴芍六君子汤甲组、柴芍六君子汤乙组、柴芍六君子汤丙组,每组8只。模型组及各给药组采用束缚法叠加四氯化碳皮下注射法造模(5 mL/kg,体重,2次/7天),同时灌胃给药,连续42天。于光镜下观察肝组织病理切片;采用全自动生化分析仪检测血清肝功能生化指标;采用比色法检测5h-尿木糖排泄率;采用TUNEL法检测肝细胞凋亡指数(apoptotic index,AI);采用RT-PCR法及Western blot法分别检测肝组织B细胞淋巴瘤-2相关X蛋白(Bcl-2-associated X protein,Bax)、B细胞淋巴瘤-2(B-cell lymphoma-2,Bcl-2)基因及蛋白的表达。结果模型大鼠血清丙氨酸转氨酶(alanine aminotransferase,ALT)、天冬氨酸氨基转移酶(aspartate aminotransferase,AST)、碱性磷酸酶(alkaline phosphatase,ALP)显著升高,白蛋白(albumin,ALB)显著降低,肝组织存在较严重病理损伤;模型大鼠AI、Bax及Bax/Bcl-2均显著高于正常组,Bcl-2及5h-尿木糖排泄率对比正常组显著降低,“知肝传脾”有其生物学依据且与肝细胞凋亡相关。各干预方式均可不同程度减轻模型大鼠上述指标变化。“当先实脾”之柴芍六君子汤丙组对该模型各指标的调节作用优于其它干预方式。结论“见肝之病,知肝传脾,当先实脾”有其生物学依据,肝细胞凋亡为“见肝之病,知肝传脾,当先实脾”的生物学机制之一。
Objective To search the biological basis of the theory that“seeing the liver disease,knowing its tendency to spread into the spleen,the spleen should be strengthened first”.To investigate the effect of chronic superimposition on liver depression and hepatocyte apoptosis in liver injured rat model.Methods A total of 48 healthy SD rats,were randomly divided them into 6 groups with 8 rats in each group.There were 6 groups in normal group,model group,dicyclol tablets group,group A,group B and group C of liujunzi decoction of paeonia lactiflorum,respectively.The model group and each treatment group were modeled by binding superposition subcutaneous injection of carbon tetrachloride(5ml/kg,b.w.,twice/7d),and administered orally for 42 consecutive days.Pathological sections of liver tissue were observed under light microscope.The biochemical indexes of serum liver function were detected by automatic biochemical analyzer.Pathological sections of liver tissue were observed under light microscope.The excretion rate of xylose in urine was detected by colorimetry.Apoptosis index(AI)was detected by TUNEL assay.The expression of Bcl-2-associated X protein(Bax),B-cell lymphoma-2(Bcl-2)gene and protein in liver tissues were detected by RT-PCR and Western Blot.Results Serum alanine aminotransferase(ALT),aspartate aminotransferase(AST)and alkaline phosphatase(ALP)in the model rats increased significantly and albumin(ALB)decreased significantly;Severe pathological damage was found in liver tissue.The AI,Bax and Bax/Bcl-2 of the model rats were all significantly higher than those of the normal group and the Bcl-2 and 5 h-urinary xylose excretion rate were lower than that of the normal group.“Knowing the tendency of spreading its disease into the spleen”has biological basis and is related to hepatocyte apoptosis.All the intervention methods can alleviate the above-mentioned changes in the model rats in the varying degrees.The intervention effect of the Bupleurum and Peony Six Gentlemen Decoction C group on the indexes of the
作者
郑智礼
史兴华
于瀚
张亿
高艺宁
魏伟莉
邓秀兰
ZHENG Zhili;SHI Xinghua;YU Han;ZHANG Yi;GAO Yining;WEI Weili;DENG Xiulan(School of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing 100029,China)
出处
《环球中医药》
CAS
2020年第2期199-206,共8页
Global Traditional Chinese Medicine
基金
国家自然科学基金面上项目(81573963)。
关键词
知肝传脾
当先实脾
肝功能
5h-尿木糖排泄率
凋亡指数
B细胞淋巴瘤-2
B细胞淋巴瘤-2相关X蛋白
Knowing the tendency of liver disease spreading to the spleen
Strengthen the spleen first
Liver function
5h-urinary xylose excretion rate
Apoptotic index
B-cell lymphoma-2
Bcl-2-associated X protein
