摘要
目的:探讨微小RNA(microRNA,miR)-655在上皮性卵巢癌中的表达、作用及其可能的分子机制。方法:采用实时定量-PCR检测miR-655在40对上皮性卵巢癌组织和正常组织中的表达,分析miR-655表达水平与临床病理之间的关系。Cell Counting Kit-8(CCK-8)和流式细胞术检测miR-655对卵巢癌细胞增殖及凋亡的影响。Western blotting检测PI3K、AKT和mTOR蛋白的表达。结果:miR-655在上皮性卵巢癌组织中的表达量明显低于正常组织(P<0.01)。miR-655表达水平在不同肿瘤直径、病理分化程度和是否淋巴结转移病人间差异均有统计学意义(P<0.05~P<0.01),而不同年龄、CA-125病人间miR-655 mimics表达差异均无统计学意义(P>0.05)。转染miR-655 mimics可明显抑制卵巢癌细胞的增殖并促进细胞的凋亡(P<0.01);miR-655 mimics转染明显抑制卵巢癌细胞中PI3K、AKT和mTOR活性(P<0.01)。结论:miR-655在卵巢癌组织中明显低表达,miR-655低表达可能与卵巢癌的肿瘤生长和分化程度相关,miR-655可明显抑制卵巢癌细胞的增殖,并促进其凋亡,其在卵巢癌病人中具有一定的靶向治疗价值。
Objective:To investigate the expression level,function and possible molecular mechanism of microRNA(miR)-655 in epithelial ovarian cancer.Methods:The expression levels of miR-655 in 40 cases of epithelial varian cancer tissues and normal tissues were detected using real-time quantitative-PCR,and the relationship between miR-655 expression and clinicopathological features was analyzed.The cell counting Kit-8(CCK-8)and flow cytometry were used to detect the effects of miR-655 on the proliferation and apoptosis of ovarian cancer cells.Western blotting was used to detect the expression of PI3 K,AKT and mTOR.Results:The expression level of miR-655 in epithelial ovarian carcinoma was significantly lower than that in normal tissues(P<0.01).The differences of the expression levels of miR-655 among patients with different tumor diameter,degree of pathological differentiation and lymph node metastasis were statistically significant(P<0.05 to P<0.01),and the differences of the expression levels of miR-655 among patients with different ages and CA-125 were not statistically significant(P>0.05).The transfection of miR-655 mimics could significantly inhibit the proliferation and promote apoptosis of ovarian cancer cells(P<0.01).The transfection of MiR-655 mimics significantly could significantly inhibit the expression of PI3 K,AKT and mTOR in ovarian cancer cells(P<0.01).Conclusions:The low expression of miR-65 in ovarian cancer tissue is significant,which may be related to the growth and differentiation of ovarian cancer.miR-655 can significantly inhibit the proliferation and induce apoptosis of ovarian cancer cells.miR-655 has a certain value of target therapy in ovarian cancer patients.
作者
马方昊
靳丽杰
叶国柳
杜丹丽
MA Fang-hao;JIN Li-jie;YE Guo-liu;DU Dan-li(Department of Gynaecological Oncology,The First Affiliated Hospital of Bengbu Medical College,Bengbu Anhui 233004,China)
出处
《蚌埠医学院学报》
CAS
2020年第2期181-184,共4页
Journal of Bengbu Medical College
