摘要
目的探讨体外受精/单精子卵浆内注射(IVF/ICSI)周期第3天(D3)低评分胚胎的临床应用价值。方法选取2016年1月至2019年1月徐州市妇幼保健院IVF/ICSI治疗周期中D3低评分的1602枚胚胎进行囊胚培养,分析囊胚形成率及可利用囊胚玻璃化冻融的临床妊娠结局。结果囊胚形成率34.96%(560/1602),可利用囊胚形成率33.4%(535/1602),冷冻囊胚535个,复苏102周期,共复苏囊胚142个,存活囊胚140个,复苏率98.60%,平均ET数1.39,临床妊娠率40.20%(41/102),种植率29.28%(41/140),流产率2.43%(1/41)。结论低评分胚胎继续培养能最大限度减少胚胎浪费,增加单次取卵累积妊娠率。同时为IVF实验室D3低评分胚胎发育潜能评价标准的制定提供理论依据。
Objective To explore the clinical application value of low score embryo on the third day of IVF/ICSI cycle(D3).Methods From January 2016 to January 2019,1602 embryos with low D3 score in IVF/ICSI treatment cycle of Xuzhou maternal and child health hospital were selected for blastocyst culture,and the blastocyst formation rate and clinical pregnancy outcome of vitrification and thawing of blastocysts were analyzed.Results The blastocyst formation rate was 34.96%(560/1602),and the usable blastocyst formation rate was 33.4%(535/1602).535 frozen blastocysts,102 cycles of resuscitation,142 blastocysts were resuscitated,140 survived blastocysts,the recovery rate was 98.60%.The average et number was 1.39.The clinical pregnancy rate was 40.20%(41/102),the planting rate was 29.28%(41/140),and the abortion rate was 2.43%(1/41).Conclusion The continuous culture of low score embryos can reduce the waste of embryos and increase the cumulative pregnancy rate of single oocyte collection.At the same time,it provides a theoretical basis for the establishment of the evaluation standard of D3 low score embryo development potential in IVF laboratory.
作者
严为巧
刘璐
宋雪梅
张雪
YAN Weiqiao;LIU Lu;SONG Xuemei;ZHANG Xue(Xuzhou Maternal and Child Health Hospital,Xuzhou,Jiangsu 221009,China)
出处
《大医生》
2019年第19期84-85,共2页
Doctor
基金
江苏省妇幼健康科研项目(编号F201626)。
关键词
低评分胚胎
囊胚培养
玻璃化冷冻
low score embryo
blastocyst culture
vitrification
