摘要
【目的】探明福建特色茶叶中的细菌群落结构特征,以及细菌群落结构在不同种类茶叶间的分布情况。【方法】采集福建特色茶叶大红袍、铁观音、花茶共15组样品,通过16S rRNA高通量测序,对不同种类茶叶中细菌的群落结构进行解析。【结果】经过高通量测序以及统计分析,最终共获得11 472个OTU,包括37个门,95个纲,150个目,207个科,277个属。比较3种茶叶,发现大红袍在物种丰度上要高于铁观音与花茶,花茶的物种多样性要高于大红袍与铁观音,且同为乌龙茶的大红袍与铁观音在细菌群落结构上更为相似。在属水平上,10组大红袍样本中共有的优势属为乳球菌属、双歧杆菌属及拟杆菌属,2组花茶样本中共有的优势属为拟杆菌属,3组铁观音中共有的优势菌属为乳球菌属与双歧杆菌属。其中,丰度前10的优势属中有5个为乳酸菌。通过共现网络分析,表明乳酸菌与其显著相关的大部分物种呈互利生长的关系。【结论】大红袍与铁观音的优势菌纲为芽孢杆菌纲和放线菌纲,花茶的优势菌纲为梭状芽胞杆菌纲与拟杆菌纲。比较3种茶叶的细菌群落结构,发现茶叶种类可能是影响细菌群落结构变化的重要因素之一,且乳酸菌在3种茶叶中均为主要优势细菌。
【Objective】 Diversity and distribution of the microbial communities in the specialty teas produced in Fujian were studied. 【Method】 Fifteen groups of well-known local teas in the province were collected for an analysis based on the highthroughput sequencing targeting 16 S rRNA gene. 【Result】 A total of 11 472 OTUs encompassing 37 phyla, 95 classes, 150 orders, 207 families, and 277 genera were isolated from the specimens. In the 3 categories, Dahongpao had the greatest richness on species;the Huacha was the most diversified;and, as oolong teas, Dahongpao and Tieguanyin had a similar microbial structure. In 10 groups of Dahongpao samples, the dominant genera were Lactococcus, Bifidobacterium, and Bacteroides;that in 2 groups of Huacha, Bacteroides;and those in 3 groups of Tieguanyin, Lactococcus and Bifidobacterium. Five of the top 10 dominant genera in the teas were lactic acid bacteria. According to the co-occurrence analysis on network, the lactic acid bacteria exhibited a synergistic growth relationship with most of the other significantly associated species. 【Conclusion】 Bacilli and Actinobacteria were the predominant bacteria found in Dahongpao and Tieguanyin, while Clostridia and Bacteroidia in Huacha. A bacterial taxonomy analysis indicated that tea category differentiated the structure of their bacterial communities.Overall, lactic acid bacteria dominantly existed in these teas produced in Fujian.
作者
何肖云
饶秋华
刘兰英
傅建炜
HE Xiao-yun;RAO Qiu-hua;LIU Lan-ying;FU Jian-wei(Institute of Quality Standards&Testing Technology for Agro-products,Fujian Academy of Agricultural Sciences/Fujian Key Laboratory of Agro-products Quality&Safety,Fuzhou,Fujian 350003,China)
出处
《福建农业学报》
CAS
CSCD
北大核心
2020年第1期28-37,共10页
Fujian Journal of Agricultural Sciences
基金
福建省科技计划公益类专项(2017R1018-1)
福建省农业科学院青年人才创新基金(YC2018-2)
福建省财政专项——福建省农业科学院科技创新团队建设项目(STIT2017-1-12)。
