摘要
目的从两色金鸡菊中制备黄诺玛苷和马里苷单体,并检测其均匀性。方法称取两色金鸡菊花蕾用55%乙醇回流提取,得到醇提物(Ethanol Extract,ETE),按乙酸乙酯∶ETE(1∶1)萃取,得到乙酸乙酯萃余物(Acetic Ether Extracted Residuum,AR),将AR用正丁醇萃取,得到的有机层经聚酰胺柱洗脱得到黄诺玛苷和马里苷,采用重结晶法纯化,经TLC法进行定性鉴别,采用HPLC法测定含量,经核磁鉴别其化学结构,根据标准样品指导原则检测马里苷和黄诺玛苷均匀性。结果从两色金鸡菊中制备出黄诺玛苷和马里苷,黄诺玛苷和马里苷重结晶之后纯度达到98.4%以上,符合标准样品纯度要求,且制备的黄诺玛苷和马里苷均匀性良好。结论本方法操作简便、工艺稳定,适用于从两色金鸡菊中制备马里苷和黄诺玛苷。
Objective To isolate and purify flavanomarein and marein from Coreopsis trinctoria Nutt and to test their homogeneity effect.MethodsCoreopsis trinctoria Nutt were refluxed with 55%ethanol to extract ethanol extract(ETE),and followed by extraction with ethyl acetate:ete(1∶1),ethyl acetate extract residuum(AR)was obtained.AR was extracted with n-butanol,and the organic layer was eluted with polyamide column to obtain flavonol glycoside and malidine,which were purified by recrystallization and thin-layer chromatography(TLC)Qualitative identification,high performance liquid chromatography(HPLC)method was used to determine the content,the chemical structure was identified by nuclear magnetic resonance(NMR),and the homogeneity of malidin and flavonoloside was detected according to the guidelines of standard samples.ResultsFlavanomarein and marein were isolated from the Coreopsis tinctoria.Their purity beyond 98.4%by the recrystallization.The homogeneity of flavanomarein and marein were excellent.ConclusionThe method established and described here was simple and stable,and thus suitable for the preparation of flavanomarein and marein from Coreopsis tinctoria.
作者
张永威
李新霞
王敬伟
李琳琳
陶义存
骆新
王丽凤
毛新民
ZHANG Yongwei;WANG Jingwei;LI Xinxia;LI Linlin;Tao Yicun;LUO Xin;WANG Lifeng;MAO Xinmin(College of Pharmacy,Xinjiang Medical University,Urumqi 830011,China;College of Basic Medical,Xinjiang Medical University,Urumqi 830011,China;College of Traditional Chinese Medicine, Xinjiang Medical University, Urumqi 830011, China)
出处
《新疆医科大学学报》
CAS
2020年第3期340-343,共4页
Journal of Xinjiang Medical University
基金
国家自然科学基金(U1303233)。
关键词
两色金鸡菊
聚酰胺柱层析
黄诺玛苷
马里苷
均匀性
Coreopsis tinctoria
polyamidecolumnchromatography
flavanomarein
marein
homogeneity
