摘要
基于响应面法对连翘果实总黄酮的闪式提取工艺条件进行研究,通过单因素试验比较了连翘果实试液液料比、乙醇浓度、闪式提取时间及闪式提取电压对连翘果实总黄酮提取率的影响,然后利用响应面法建立模型进行分析,得出连翘果实总黄酮闪式提取工艺的最优参数并进行验证。结果表明,在连翘果实试液液料比、闪式提取时间和闪式提取电压分别在27∶1、80 s和109 V时能达到最优提取条件;在此条件下,连翘果实总黄酮提取率最高,可达18.256%,重复验证得到连翘果实黄酮提取率的实际均值为18.030%,接近理论值,说明获得的最佳工艺参数准确可靠。研究结果可为连翘果实中黄酮的工业化提取提供一定的理论参考。
In this experiment,the response surface method was used to optimize the flash extraction conditions of flavonoids in Forsythia suspensa fruit.The effects of liquid-to-solid ratio,ethanol concentration,flash extraction time and flash extraction voltage on the extraction rate of total flavonoids in Forsythia suspensa fruits were compared by single factor test.Then the model was established by response surface method,and the optimal parameters of the flash extraction process of total flavonoids in Forsythia suspensa fruit were verified.The results showed that the optimum extraction conditions could be achieved at 27∶1,80 s and 109 V in the test of liquid-to-solid ratio,flash extraction time and flash extraction voltage of Forsythia suspensa fruit,respectively.The extraction rate of flavonoids was the highest,reaching 18.256%,and the actual average value of total flavonoids extraction rate of Forsythia suspensa fruit was 18.030%,which was close to the theoretical value.So,the optimal process parameters were accurate and reliable.The research results will provide a theoretical reference for the industrial extraction of total flavonoids in the fruit of Forsythia suspensa fruit.
作者
邱相坡
梁璇
王文斌
QIU Xiangpo;LIANG Xuan;WANG Wenbin(College of Life Sciences,Shanxi Agricultural University,Taigu 030801,China)
出处
《山西农业科学》
2020年第3期452-457,共6页
Journal of Shanxi Agricultural Sciences
基金
山西省基础研究计划项目(201801D121204)
山西农业大学国家自然科学基金培育项目(2017GPY06)
山西农业大学科技创新基金项目(2018yz003)
山西农业大学博士科研启动基金项目(XB2009002)。
关键词
连翘果实
闪式提取
总黄酮
响应面法
Forsythia suspensa fruit
flash extraction
total flavonoids
response surface method
