摘要
目的探讨Ki67和异柠檬酸脱氢酶(IDH)在老年(年龄≥65岁)胶质母细胞瘤(GBM)中的表达以及性别、卡氏(KPS)评分、切除程度和放化疗等临床因素与老年GBM患者预后之间的潜在关系。方法对象为郑州大学第一附属医院2013至2018年间收治的老年GBM患者,共54例。采用链霉卵白素-过氧化物酶法(SP)检测Ki67表达情况,采用Sanger测序的方法检测IDH突变情况。最终进行统计学分析以确定Ki67指数、IDH突变、性别、KPS评分、切除程度以及放化疗是否与患者的临床预后具有相关性。结果54例老年GBM患者均没有检测到IDH突变。单因素分析表明,Ki67指数(P=0.033)、KPS评分(P=0.008)和切除程度(P<0.001)是与老年GBM患者预后相关的影响因素之一。术后是否进行辅助放化疗与患者的预后具有明显相关性,进行放疗(P=0.002)和化疗(P=0.034)的患者具有更长的生存期。而性别(P=0.467)与本组患者的预后没有明显相关性。多因素分析表明,放疗(OR值2.446,P=0.009)及切除程度(OR值6.976,P<0.001)是老年GBM患者预后的独立预测因子。结论IDH突变在老年GBM中确实罕见,提示老年GBM患者往往具有预后不良的分子病理学表型。Ki67、KPS评分、切除程度和放化疗是影响患者预后的因素。
Objective This study was designed to investigate prognostic factors and the potential relationship between the expression of Ki67 and IDH of glioblastoma in the elderly(≥65 years old)and the clinical factors such as gender,the KPS score and treatments including surgical resection,radiotherapy and chemotherapy and the prognosis of such patients.Methods Fifty-four elderly patients(≥65 years old)with glioblastoma admitted to the First Affiliated Hospital of Zhengzhou University from 2013 to 2018 were enrolled in this study.The expression of Ki67 was detected by immunohistochemical SP method and the mutation of IDH was detected by Sanger sequencing.Finally,statistical analysis was performed to determine whether Ki67,the mutation of IDH,gender,the KPS score and the extent of resection,radiotherapy and chemotherapy were associated with the clinical prognosis of the patients.Results Of the 54 elderly patients with glioblastoma,none was detected with IDH mutation.Univariate analysis showed that Ki67(P=0.033),the KPS score(P=0.008),the extent of resection(P<0.001)were factors influencing the prognosis of elderly patients with glioblastoma.Patients receiving postoperative adjuvant radiotherapy(P=0.002)and chemotherapy(P=0.034)had longer survival time.There was no significant correlation between gender(P=0.467)and prognosis.Multivariate analysis demonstrated that radiotherapy(OR 2.446,P=0.009)and the extent of resection(OR 6.976,P<0.001)were independent prognostic factors.Conclusions No IDH mutation was detected in all the patients in this study,indicating that IDH mutation is indeed rare in elderly glioblastoma,which suggests that geriatric population often harbor a molecular phenotype with poor prognosis.Ki67,KPS score,the extent of resection,radiotherapy and chemotherapy were the factors influencing the prognosis of patients.
作者
王艳敏
段文超
王伟伟
赵亚鹏
刘献志
张振宇
Wang Yanmin;Duan Wenchao;Wang Weiwei;Zhao Yapeng;Liu Xianzhi;Zhang Zhenyu(Department of Neurosurgery,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450000,China;Department of Pathology,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450000,China)
出处
《中华医学杂志》
CAS
CSCD
北大核心
2020年第2期121-124,共4页
National Medical Journal of China
基金
国家自然科学基金(81702465,U1804172)。
