摘要
目的为研究评价通体结香技术产沉香(通体香)的慢性毒性。方法本研究参考《中国药典》沉香用法用量,对SD大鼠连续90 d分别饲喂药用剂量组(1262.4、631.2和157.8 mg·kg^-1)和食用剂量组(150、105和45 mg·kg^-1)的通体香提取物,并连续记录大鼠的体重增加量和食物利用率,90 d后,处死大鼠,采集大鼠血液和尿液,检测血液指标、血清生化指标和尿液指标,并对大鼠重要内脏器官进行解剖称重和病理学组织检查。结果以通体香提取物连续饲喂SD大鼠90 d,大鼠体重增重、食物利用率、血液学指标、血清生化指标、尿液指标和内脏器官重量与对照组相比无显著性差异(P>0.05),组织学检查结果表明,雄、雌大鼠实验组与对照组均有部分病变,但药用剂量组和食用剂量组高剂量组的病变例数与对照组相比均无显著性差异,说明病变的发生与通体香提取物无关。结论通体结香技术所产沉香对实验动物安全无慢期毒性,即作为药用和食用均无慢性毒性。
OBJECTIVE To assess the chronic toxicity of agarwood extracts produced by agar-wit.METHODS Agarwood usage in Chinese Pharmacopoeia was referred.SD rats were used as the experimental animal and fed with agarwood extracts at pharmaceutical dosages(1262.4,631.2 and 157.8 mg·kg^-1)and functional food dosages(150,105 and 45 mg·kg^-1)for 90 d.The weight and food utilization were recorded.The rats were killed on the 90th day,blood and urine were collected for routine blood,serum biochemical indicators and urine index tests,and the bodies were dissected for histological examination.RESULTS The two groups of rats showed same results.The body weight,food utilization,routine blood,serum biochemical indicators and urine index were not significantly changed compared with the control group(P>0.05).Histological examination showed that the male and female rats both had some lesions,but the group of high dose extracts had no significant difference compared with the control group,also indicating that the lesions had no relationship with agarwood extracts.CONCLUSION The agarwood extracts produced by agar-wit have no chronic toxicity for mammal in regards of body weight,biochemical indicators and pathology at both pharmaceutical dosages and functional food dosages.
作者
侯文成
王灿红
冯剑
杨云
刘洋洋
魏建和
HOU Wen-cheng;WANG Can-hong;FENG Jian;YANG Yun;LIU Yang-yang;WEI Jian-he(Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine&Key Laboratory of State Administration of Traditional Chinese Medicine for Agarwood Sustainable Utilization,Hainan Branch of the Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Haikou 570311,China;Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine,Ministry of Education&National Engineering Laboratory for Breeding of Endangered Medicinal Materials,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193,China)
出处
《中国药学杂志》
CAS
CSCD
北大核心
2019年第23期1970-1975,共6页
Chinese Pharmaceutical Journal
基金
国家重点研发计划项目资助(2018YFC1706400)
中组部“万人计划”项目资助(99950534)
中国医学科学院医学与健康科技创新工程项目资助(2016-I2M-2-003)
