摘要
目的研究云南省普洱市蝙蝠携带的乙肝病毒多样性及流行情况.方法在普洱市采集84只蝙蝠肝组织样本,通过高通量测序、特异性PCR方法和基因移步法对蝙蝠乙肝病毒进行检测和扩增,并进行序列分析和系统发育分析.结果3.75%(3/84)的蝙蝠携带乙肝病毒,均来自于中华菊头蝠,命名为PuEr BHBV.PuEr BHBV的基因结构、保守功能结构域与其他乙肝病毒高度相似.序列分析显示,PuEr BHBV与河南内乡发现的NeiXiang-Rp-89的核苷酸和氨基酸同源性分别为87.1%和90.5%.系统进化分析显示,蝙蝠乙肝病毒呈现丰富的多样性,蝙蝠乙肝病毒分为两个较远的进化分支.同时,PuEr BHBV与贵州、河南内乡蝙蝠携带乙肝病毒具有最近的亲缘关系,但与巴拿马的TBHBV处在不同的进化分支.结论PuEr BHBV是一株新的蝙蝠乙肝病毒株,病毒在不同蝠种之间的传播和地域差别可能共同促进了蝙蝠乙肝病毒的进化,本研究丰富了蝙蝠乙肝病毒的多样性.
Objective To understand the diversity and prevalence of bat hepatitis B virus in Yunnan province.Methods A total of 84 bat tissue samples were collected from Pu'er area.High-throughput sequencing,specific PCR and genome walking method were used to amplify bat hepatitis B virus nucleic acids.The sequences analysis and phylogentic analysis were performed.Results The analyses showed that 3.75%(3/84)of the bats carried hepatitis B virus and all positive sameples were from Rhinolophus sinicus.The virus strains were named as PuEr BHBV.Sequence analysis showed that PuEr BHBV and NeiXiang-Rp-89 had 87.1%nucleotide similarity and 90.5%amino acid similarity.Phylogenetic analysis indicated rich diversity of the bat hepatitis B virus and the strains were separated into two branches.The PuEr BHBV was clustered with bat hepatitis B virus discovered from Guizhou province,Neixiang of Henan province,but clustered in different branch from the stains isolated from Panama.Sequence analysis showed that PuEr BHBV present similar gene structure and conserved functional domain with other hepatitis B viruses.Conclusions PuEr BHBV should be considered as a new bat hepatitis B virus strain.Virus transmissions among different bat species and different geographic area may have driven the adaptive evolution of bat the virus,leading to the genetic diversity of the bat hepatitis B virus.This study extends our knowledge of the genetic diversity of hepatitis B virus.
作者
徐亚陇
王静林
李利利
段招军
郭科
马超锋
Xu Yalong;Wang Jinglin;Li Lili;Duan Zhaojun;Guo Ke;Ma Chaofeng(School of Public Health,Shaanxi University of Traditional Chinese Medicine,Xi'an 712046,China;Yunnan Tropical and Subtropical Animal Virus Disease Key Laboratory,Yunnan Anim al Science and Veterinary Institute,Kunming 650224,China;National Institute for Viral Disease Control and Prevention,China CDC,Beijing 100052,China;School of Basic Medical Sciences,Anhui Medicine University,Hefei 230031,China)
出处
《国际病毒学杂志》
2019年第6期416-422,共7页
International Journal of Virology
基金
国家自然科学基金(31460669)
国家重大科技专项(2018ZX10305-409-004-002)。
关键词
蝙蝠
乙肝病毒
云南普洱
遗传多样性
Bat
Hepatitis B virus
Pu'er area
Yunnan province
Genetic diversity
