摘要
目的:建立胃内容物中甲卡西酮及其代谢物卡西酮的LC-MS/MS检验方法。方法:以双苯戊二氨酯(SKF525A)为内标,Column Eclipse Plus C18色谱柱(1.8μm, 100 mm×3 mm)为固定相,流动相为0.1%甲酸水溶液和乙腈,梯度洗脱,流速为0.3m L/min,进样量为1μL。通过电喷雾离子源(ESI),利用正离子多反应监测模式(MRM)进行检测。结果:胃内容物中甲卡西酮检验方法线性范围为1-5000 ng/g,标准曲线方程Y=0.026X+0.4501(R2=0.9998),检出限为0.5 ng/g,定量限为1.0 ng/g;卡西酮的检验方法线性范围为2-5000 ng/g,标准曲线方程Y=0.0099X+0.1576(R2=0.9997),检出限为1.0 ng/g,定量限为2.0 ng/g。结论:该方法操作简便,检测灵敏度较高,可用于甲卡西酮中毒案件胃内容物中甲卡西酮及其代谢物卡西酮的检验。
Objective: To establish a liquid chromatography-tandem mass spectrometry(LC-MS/MS) method for the determination of methcathinone and its metabolite cathinone in gastric contents. Methods: Proadifen hydrochloride(SKF525 A) was used as internal standard. Column Eclipse Plus C18 column(1.8 μm, 100 mm×3 mm)was used as stationary phase. Mobile phase was 0.1 % formic acid aqueous solution and acetonitrile, gradient elution with flow rate of 0.3 m L/min and injection volume of 1 μL. Electrospray ion source(ESI) was detected by positive ion multi reaction monitoring mode(MRM). Results: The linear range of methcathinone in gastric contents was 1-5000 ng/g, and the standard curve equation was Y=0.026 X+0.4501(R2=0.9998), the detection limit was 0.5 ng/g, and the quantitative limit was 1.0 ng/g. The linear range of cathinone in gastric contents was 2-5000 ng/g, and the standard curve equation was Y=0.0099 X+0.1576(R^2=0.9997), the detection limit was 1.0 ng/g, and the quantitative limit was 2.0 ng/g. Conclusions: The established method for the detection of methcathinone and its metabolite cathinone in the gastric contents is simple and sensitive. This method can be used for the detection of methcathinone poisoning in the public security.
作者
赵明明
张旭东
刘冬娴
张尔力
任宏宾
ZHAO Ming-ming;ZHANG Xu-dong;LIU Dong-xian;ZHANG Er-li;REN Hong-bin(Criminal Technology Department,Hunan Police Academy,Changsha,Hunan,410138,China;Changsha Public Security Bureau narcotics detachment,Changsha,Hunan,410000,China)
出处
《现代生物医学进展》
CAS
2019年第21期4098-4102,共5页
Progress in Modern Biomedicine
基金
湖南省自然科学基金项目（2018JJ2109）
湖南省教育厅科学研究重点项目(15A057)
