摘要
建立了LC-MS/MS法定性定量分析甲卡西酮。采用三重串联四极杆液质联用仪(LC/QQQ),AgilentZorbax Eclipse Plus C18色谱柱(100 mm×2.1 mm,1.8μm),流动相为0.1%甲酸-乙腈,梯度洗脱,流速为0.3mL/min。质谱应用ESI源、正离子模式、多反应监测(MRM)方式。在0.1～10 000 ng/mL质量浓度范围内线性关系良好,r2=0.999 8,日内与日间保留时间和峰面积的相对标准偏差不大于5.28%,检出限为0.04 ng/mL,回收率为95.6%～100.7%。该方法适用于甲卡西酮的定性、定量分析。
A qualitative and quantitative analysis method of methcathinone by LC-MS / MS was established. Agilent LC/QQQ and Agilent Zorbax^Eclipse Plus C18 column（100 mm × 2.1 mm, 1.8 μm） were used, mobile phase was 0.1% formic acid-acetonitrile at flow rate of 0.3 mL/min with gradient elution. ESI source was used for mass spectrometry, and positive ion mode, multiple reaction monitoring （MRM） mode were used for detecting concentration of methcathinone. This method had good linearity with the real value at the range of 0.1-10 000 ng/mL, r2=0.999 8. Both intra-day and inter-day precisions expressed by relative standard deviations of retention time and peak area were less than 5.28%. The detection limit was 0.04 ng/mL. The recovery was 95.6%-100.7%. This method is suitable for qualitative and quantitative analysis.
出处
《化学分析计量》
CAS
2013年第3期51-53,共3页
Chemical Analysis And Meterage
