摘要
目的采取固相萃取前处理方法去除补骨脂中药配方颗粒剂中影响补骨脂素和异补骨脂素测定的杂质成分,然后通过反相高效液相色谱法测定经纯化后的样品中补骨脂素和异补骨脂素含量。方法采用C18 SPE小柱纯化样品,色谱柱:Diamonsil C18(250mm×4.6mm,5μm);流动相:甲醇-水(55∶45)为流动相;流速:1mL·min-1;检测波长为245nm进行检测。结果补骨脂素回归方程:A=612134C-2897.2(r=0.9995),平均回收率99.5%,RSD=1.58%;异补骨脂素A=578853-2739.6(r=0.9993),平均回收率99.8%,RSD=1.40%。结论优化后的方法简便、准确、快速、重现性好。
OBJECTIVE The psoralen and isopsoralen were purified by solid phase extraction and determined by HPLC.METHODS C18SPE was used to purify the samples,column:Diamonsil C18(250 mm×4.6 mm,5μm);mobile phase:methanol-water(55∶45);flow rate:1 mL·min-1;detection wavelength:245 nm.RERULTS Regression equation for Psoralen:A=612134 C-2897.2(r=0.9995),average recovery rate of 99.5%for Psoralen,RSD=1.58%;Regression equation for isopsoralen:A=578853-2739.6(r=0.9993),average recovery rate of 99.8%for isopsoralen,RSD=1.40%.CONCLUSION The optimized method is simple,accurate,rapid and reproducible.
作者
李光河
齐元芝
杨戒骄
LI Guang-he;QI Yuan-zhi;YANG Jie-jiao(Shenzhen People′s Hospital,Shenzhe n 518020,China)
出处
《海峡药学》
2019年第12期73-75,共3页
Strait Pharmaceutical Journal
关键词
补骨脂中药配方颗粒剂
SPE-RP-HPLC
含量测定
Psoralea corylifolia formula granules
Solid Phase Extraction-HPLC
Psoralen
isopsoralen
Determination
