摘要
目的:观察紫朱软膏促进急性创面愈合的疗效及对自噬蛋白LC3和Becin1的影响。方法:SD大鼠背部急性皮肤创伤形成后,随机分为两组,紫朱软膏组创面外用紫朱软膏,对照组给予生理盐水外敷,比较两组创伤后4、7、14、21 d的创面愈合率,HE染色观察创面组织形态,透射电镜观察各时相点创面自噬体,免疫组化法、WB法检测自噬标志蛋白Beclin-1、LC3的表达及变化。结果:与对照组相比,治疗组愈合率高,愈合时间短,再上皮化速度快,电镜下可见自噬体减少,LC3、Beclin-1表达明显减弱。结论:紫朱软膏可能通过抑制细胞的自噬活性促进创面愈合。
Objective: To observe the effect of Zizhu ointment on acute wound healing and its effect on the expression of autophagy proteins LC3 and Becin1.Methods: SD rats with acute skin injury were randomly divided into groups. The experimental group was treated with external application of Zizhu ointment, and the control group was given external saline. The wound healing rate of the two groups was compared at 4 d, 7 d, 14 d and 21 d after trauma. The morphology of wounds was observed by HE staining, and the autophagosomes of wounds were observed by transmission electron microscopy. Expression and changes of autophagy marker proteins Beclin-1 and LC3 were detected by immunohistochemistry and WB assay. Results: Compared with the control group, experimental group had a high healing rate, shorter healing time, and a faster epithelialization rate. Under the electron microscope, autophagosomes of experimental group were reduced, and the expression of LC3 and Beclin-1 was significantly decreased. Conclusion: Zizhu ointment may promote wound healing by inhibiting autophagy activity of cells.
作者
胡啸明
黄仁燕
韩强
严仕梦
柳国斌
HU Xiao-ming;HUANG Ren-yan;HAN Qiang;YAN Shi-meng;LIU Guo-bin(Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203)
出处
《海南医学院学报》
CAS
2020年第4期251-256,261,共7页
Journal of Hainan Medical University
基金
国家自然科学基金面上项目(81774310)
国家自然科学基金青年项目(81804095)
上海中医健康服务协同创新中心项目(ZYJKFW201701002)~~
关键词
急性创面
自噬
紫朱软膏
Acute wound
Autophagy
Zizhu ointment
