摘要
目的研究23例大疱性类天疱疮(BP)患者血清中抗BP180抗体的表达水平和治疗前后的变化及抗体指数与病情严重程度的相关关系,以探讨抗BP180抗体水平在BP患者的诊断、监测病情变化及治疗中的应用。方法对23例患者通过病理及直接免疫荧光进行诊断,并对患者的病情进行评估,应用酶联免疫吸附试验(ELISA)法检测患者及20例正常人血清中抗BP180抗体的水平,最后通过统计分析判断各指标间的关系及意义。结果23例患者血清经ELISA检测抗BP180抗体阳性18例;治疗后的抗BP180抗体指数(67.94±54.73)较治疗前(74.22±59.06)下降,治疗前与治疗后的差值为3.66±12.71,差异有统计学意义(P<0.05);治疗前后抗BP180抗体指数均与病情评分有相关性(治疗前r=0.417,P<0.05;治疗后r=0.818,P<0.01)。20例正常对照组阳性1例,阴性19例。ELISA检测BP血清抗BP180抗体的灵敏度为78.26%,特异性为95%。结论BP患者血清中抗BP180抗体水平与疾病的严重程度有一定的相关性,有较高的灵敏度和特异性,可作为BP的常规辅助诊断,并用于监测病情变化及指导治疗。
Objective In order to investigate the application of anti-BP180 antibody in diagnosis and treatment of patients,the level and changes of anti-BP180 antibody in 23 patients with bullous pemphigoid and the relationship between the antibody index and the severity of the disease were analyzed.Methods Twenty-three patients were diagnosed for bullous pemphigoid by pathology and direct immunofluorescence,and the patient's pathogenetic condition was assessed.The level of anti-BP180 antibody of patients and normals was detected by enzyme-linked immunosorbent assay(ELISA).Finally,statistics was performed to analyze the relationship and significance of each index.Results Anti-BP180 antibody was positive in 18 of 23 patients by ELISA.The index of anti-BP180 antibody after treatment(67.94±54.73)was decreased compared with that before treatment(74.22±59.06),and the difference value was 3.66±12.71,the differences were statistically significant(P<0.05).The index of anti-BP180 antibody was correlated with the disease score(r=0.417,P<0.05 before treatment;r=0.818,P<0.01 after treatment).There were one positive case and 19 negative cases in 20 cases of normal controls.The sensitivity of ELISA for anti-BP180 antibody was 78.26%and specificity was 95%.Conclusion The anti-BP180 antibody level of patients with bullous pemphigoid is correlated with the severity of the disease with high sensitivity and specificity.The detection of anti-BP180 antibody level can be used for diagnosis and guidance of state and treatment of the disease.
作者
王翠芹
王艳心
余海
刘利君
李保强
Wang Cuiqin;Wang Yanxin;Yu Hai;Liu Lijun;Li Baoqiang(The Affiliated Hospital of Chengde Medical University,Chengde 067000,China;Cangzhou Hospital of Integrated Traditional Chinese and Western,Cangzhou 061000,China)
出处
《中国中西医结合皮肤性病学杂志》
CAS
2020年第1期57-59,共3页
Chinese Journal of Dermatovenereology of Integrated Traditional and Western Medicine
基金
河北省医学适用技术跟踪项目基金资助（基金编号G2015058）
