摘要
采用25个果实性状指标和19对SSR引物对四川栽培的19份李种质资源进行主成分分析和聚类分析,并以欧式距离、前3个主成分和遗传相似性为基础,分别作系统聚类、三维排序图和UPGMA聚类。结果表明:19份李种质25个果实性状指标的平均变异系数为54.71%,其中果皮红绿色差值a*值的变异系数最大,为436.14%,果核侧径的变异系数最小,为9.99%;系统聚类可将李果实分为深色系大果型和浅色系小果型;主成分分析中,前3个主成分累计贡献率为73.27%,第1主成分(果重因子)的贡献率为49.45%,第2主成分(果形与色泽因子)的贡献率为14.21%,第3主成分(营养因子)的贡献率为9.62%;以主成分PC1、PC2和PC3向量为轴的三维排序图清晰地展示出各种质在果实性状上的相对差异;SSR标记显示供试材料的遗传多样性丰富,UPGMA聚类与系统聚类和主成分三维排序的结果基本一致。
Principal component analysis and cluster analysis of 19 plum germplasm resources which planted in Sichuan were conducted using 25 fruit characters and 19 SSR markers.The plum germplasm were classified based on Euclidean distance,the first three principal components and genetic similarity,as well as systematic cluster,three-dimensional ordination plot and UPGMA clustering analysis.Results showed that the average coefficient of variation of the 25 fruit characters was 54.71%,the variation coefficient of a*value of the peel was the highest(436.14%)and the variation coefficient of stone lateral dinmeter was the lowest(9.99%).The system clustering mainly divided these varieties into large fruit with deep color,small fruit with light color.In the principal component analysis,the first three principal components account for 73.27%of variation among the varieties,the first principal component(PC1)which regarded as fruit weight factor contributed 49.45%,the second principal component(PC2)as fruit shape and the color factor contributed 14.21%,and the third principal component(PC3)as nutritional factor contributed 9.62%.The three-dimensional ordination plot with PC1,PC2 and PC3 vectors clearly showed the relative differences of the main characters for the 19 plum germplasm resources.SSR markers showed that the genetic diversity of the tested materials was rich.The results of UPGMA clustering were similar to those of systematic clustering and three-dimensional ordination plot.
作者
李雷
王海燕
陶尚玉
陈梦微
龙星雨
夏惠
祝进
邓群仙
周琼
LI Lei;WANG Haiyan;TAO Shangyu;CHEN Mengwei;LONG Xingyu;XIA Hui;ZHU Jin;DENG Qunxian;ZHOU Qiong(College of Horticulture,Sichuan Agricultural University,Chengdu,Sichuan 611130,China;Tea and Furit Technology Extension Station of Dazhou,Dazhou,Sichuan 635000,China;Institute of Pomology and Olericulture,Sichuan Agricultural University,Chengdu,Sichuan 611130,China;Sichuan Horticultural Crop Technology Extension Station,Chengdu,Sichuan 611041,China;Hanyuan County Bureau of Agriculture and Rural Affairs,Hanyuan,Sichuan 625300,China)
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2020年第1期38-47,共10页
Journal of Hunan Agricultural University(Natural Sciences)
基金
四川省科技支撑项目(2013FZ0036)
四川农业大学“双支计划”基金项目(03572783)
四川农业大学与四川省汶川县校地合作项目(1922339018)
关键词
李
果实性状
微卫星标记
聚类分析
plum
fruit characters
simple sequence repeats makers
cluster analysis
