摘要
目的采用纸喷雾质谱(paper spray ionization mass spectrometry,PSI-MS)技术,建立养殖水中孔雀石绿(malachite green,MG)的快速定量筛查方法。方法以氯仿为萃取剂,低共熔溶剂(deep eutectic solvents,DESs)为助溶剂,快速提取MG,氘代孔雀石绿(malachite green D5,MG-D5)同位素内标稀释定量,PSI-MS检测,C18色谱柱(4.6 mm×250 mm,5μm)。结果方法工作曲线范围0.1~10μg/mL(相关系数>0.999),检出限0.03μg/mL,定量限0.1μg/mL,日内精密度3.5%,日间精密度6.2%,加标回收率98.9%~106.9%(n=7),单个样品PSI-MS检测耗时1 min,检测结果与液相色谱-串联质谱法(liquid chromatography-tandem mass spectrometry,LC-MS/MS)一致。结论所建方法应用于养殖水中MG定量检测,准确可靠,较常规LC-MS/MS法简便快速、成本低。
OBJECTIVE A rapid quantitative technique had been developed for the screening of malachite green(MG) in aquaculture water using paper spray ionization mass spectrometry(PSI-MS). METHODS To rapid extract MG, chloroform and deep eutectic solvents(DESs) were used as extraction agent and co-solvent respectively. malachite green D5(MG-D5) was used as an internal standard to quantitative determination MG, C18 chromatographic column(4.6 mm×250 mm, 5 μm). RESULTS The method showed a good linearity(R2=0.999) in the concentration range of 0.1-10 μg/mL with the detection limit of 0.03 μg/mL and the quantitative limit of 0.1 μg/mL. The intra-and inter-day precisions were 3.5% and 6.2%, and the recovery ranged from 98.9% to 106.9%(n=7). The detection result by PSI-MS were in agreement with those by liquid chromatography-tandem mass spectrometry(LC-MS/MS), but the run time was only 1 min each sample. CONCLUSION The method is accurate and reliable. And compared with the conventional LC-MS/MS technology, it is simple, rapid and low-cost.
作者
曾栋
喻目千
文瑞芝
冯家力
Zeng Dong;Yu Muqian;Wen Ruizhi;Feng Jiali(Physical&Chemical Analysis Department,Hunan Provincial Center for Disease Control and Prevention,Changsha 410005,China;Changsha Food and Drug Administration,Changsha 410016,China;College of Science,Central South University of Forestry&Technology,Changsha 410004,China)
出处
《卫生研究》
CAS
CSCD
北大核心
2020年第1期107-111,共5页
Journal of Hygiene Research
基金
湖南省卫生计生委科研基金(No.C2015-064)
关键词
纸喷雾质谱
低共熔溶剂
养殖水
孔雀石绿
paper spray ionization mass spectrometry
deep eutectic solvents
aquaculture water
malachite green
