摘要
目的建立HPLC-ELSD和LC-MS/MS测定方法,对银柴颗粒进行山银花藤检查。方法以山银花藤中的特征性成分灰毡毛忍冬皂苷乙及川续断皂苷乙为指标,采用HPLC-ELSD法进行限量检查,并采用LC-MS/MS法对结果进行验证。结果在收集的24批样品中,有6批样品检出了灰毡毛忍冬皂苷乙,可能存在以山银花藤替代忍冬藤投料的情况。结论建立的检查方法准确、可靠,专属性强,可用于检查银柴颗粒中山银花藤的使用情况。
Objective To establish HPLC-ELSD and LC-MS/MS methods for the inspection of Lonicerae Caulis in Yinchai granules.Methods HPLC-ELSD was used to determine Lonicerae Caulis with macranthoside B and dipsacoside B as the control.LC-MS/MS was used to verify the results.Results Among the 24 sample batches measured,macranthoidin B was detected in 6 batches,indicating Lonicerae Caulis might be used in Yinchai granules.Conclusion The method is accurate,specific and reliable,which can be used to detect Lonicerae Caulis in Yinchai granules.
作者
黄晓燕
罗时
李荣玮
李瑞莲
HUANG Xiao-yan;LUO Shi;LI Rong-wei;LI Rui-lian(Hunan Institute for Drug Control,Changsha 410001;Hunan Engineering&Technology Research Center for Pharmaceutical Quality Evaluation,Changsha 410001)
出处
《中南药学》
CAS
2019年第12期2119-2122,共4页
Central South Pharmacy
基金
国家药品监督管理局2018年度国家药品抽验任务（编号：50）
