摘要
为了探究4-羟基-3-甲氧基肉桂酸乙酯的除草作用方式,在前期蛋白组学研究结果的基础之上,对4-羟基-3-甲氧基肉桂酸乙酯的潜在除草靶标基因进行筛选。利用实时定量PCR(RT-qPCR)技术,测定茎叶喷施质量浓度为1000 mg/L的4-羟基-3-甲氧基肉桂酸乙酯0,15,30,45 min后,拟南芥野生型株系和6种T-DNA插入株系的拟南芥中AT2G43030和AT1G66240基因的相对表达量,并比较拟南芥野生型株系和6种T-DNA插入株系的受损程度,分析AT2G43030和AT1G66240基因在4-羟基-3-甲氧基肉桂酸乙酯除草过程中的作用。结果表明:相比野生型拟南芥株系而言,AT2G43030和AT1G66240基因的6种T-DNA插入纯合株系对4-羟基-3-甲氧基肉桂酸乙酯更为敏感,茎叶喷施4-羟基-3-甲氧基肉桂酸乙酯24 h后不同T-DNA株系的叶片损伤程度相较野生型株系受损更为严重,证明AT2G43030和AT1G66240基因与拟南芥对4-羟基-3-甲氧基肉桂酸乙酯的防御系统有关,同时也说明4-羟基-3-甲氧基肉桂酸乙酯的除草作用机制与植株的光合作用和细胞膜功能有关。
In order to explore the herbicidal action of 4-hydroxy-3-methoxy cinnamic acid ethyl,the potential herbicidal target gene of ethyl 4-hydroxy-3-methoxy cinnamic acid ethyl was screened,based on previous results of proteomics studies.Real-time quantitative PCR(RT-qPCR)was used to determine,the relative quantitative expression levels of AT2G43030 and AT1G66240 genes of wild-type and six T-DNA insertion lines of A.thaliana,which were sprayed with 1000 mg/L 4-hydroxy-3-methoxy cinnamic acid ethyl for 0,15,30 and 45 min,respectively.The damage degrees of wild-type and other six T-DNA insertion lines of A.thaliana were compared,and the roles of AT2G43030 and AT1G66240 genes in the herbicidal process of 4-hydroxy-3-methoxy cinnamic acid ethyl were analyzed.The results showed that the six T-DNA insertion homozygous lines with AT2G43030 and AT1G66240 genes were more sensitive to 4-hydroxy-3-methoxy cinnamic acid ethyl,and the degree of leaf damage in different T-DNA strains sprayed with 4-hydroxy-3-methoxy cinnamic acid ethyl for 24 h was more severe,compared with the wild-type A.thaliana,which proved that the AT2G43030 and AT1G66240 genes were related to the defense system of Arabidopsis to 4-hydroxy-3-methoxy cinnamic acid ethyl.This indicated that the mechanism of herbicidal action of 4-hydroxy-3-methoxy cinnamic acid ethyl was related to photosynthesis and cell membrane function of plants.
作者
王稳
靳丽宇
张利辉
WANG Wen;JIN Liyu;ZHANG Lihui(College of Plant Protection,Hebei Agricultural University,Baoding 071001,China)
出处
《华北农学报》
CSCD
北大核心
2020年第1期168-174,共7页
Acta Agriculturae Boreali-Sinica
基金
河北省自然科学基金项目(C2017204050)
国家自然科学基金项目(31401759)
国家重点研发课题(2018YFD0200607)
