摘要
为研究醋酸钙不动杆菌的致病机制及防控措施,对疑似患有醋酸钙不动杆菌的病死牛肺脏组织进行病原分离,得到了1株优势菌命名为9-1。对分离菌株进行形态学观察、生化试验、16S rDNA基因序列测定,动物致病性试验和药敏试验。结果显示,分离菌株为革兰阴性杆菌且对试验小鼠具有较强的致病性,经生化试验和16S rDNA基因序列测定分析确定为醋酸钙不动杆菌。药敏试验结果显示该分离菌株对氟苯尼考、头孢噻肟、头孢氨苄、阿奇霉素耐药,对其余8种测试药物敏感。经β-内酰胺类(TEM、DHA、OXA-23、OXA-58)耐药基因检测结果显示均为阴性。为醋酸钙不动杆菌引起的疾病选择合理的抗菌药物提供了一定科学依据,并为进一步研究其耐药机制奠定了基础。
In order to study the pathogenic mechanism and prevention and control measures of Acinetobacter calcoaceticus,the pathogen isolation of the diseased dead bovine lung tissue suspected of having Acinetobacter calcoaceticus was obtained,and one dominant bacteria was named 9-1.The isolates were subjected to morphological observation,biochemical test,16S rDNA gene sequence determination,animal pathogenicity test and drug susceptibility test.The results showed that the isolated strain was Gram-negative bacillus and had strong pathogenicity to the test mice.It was identified as Acinetobacter calcoaceticus by biochemical test and 16S rDNA gene sequence analysis.The drug susceptibility test showed that the isolated strain was resistant to florfenicol,cefotaxime,cephalexin,and azithromycin,and was sensitive to the other eight test drugs.The results ofβ-lactam(TEM,DHA,OXA-23,OXA-58)drug resistance genes were all negative.This test provides a scientific basis for the selection of reasonable antibacterial drugs for diseases caused by Acinetobacter calcoaceticus,and lays a foundation for further research on its resistance mechanism.
作者
李晨
王思月
嵇少泽
李雪嵩
张喜庆
田佳琪
马红霞
高云航
LI Chen;WANG Siyue;JI Shaoze;LI Xuesong;ZHANG Xiqing;TIAN Jiaqi;MA Hongxia;GAO Yunhang(College of Animal Science and Technology,Jilin Agricultural University, Changchun 130118, China)
出处
《华北农学报》
CSCD
北大核心
2019年第S01期318-323,共6页
Acta Agriculturae Boreali-Sinica
基金
畜禽重要病原菌耐药性检测技术研究(2016YFD0501301)
新型畜禽药创制与产业化(2016YFD0501401)
关键词
醋酸钙不动杆菌
分离鉴定
药敏试验
耐药基因
Acinetobacter calcoaceticus
Isolation and identification
Drug sensitive test
Resistance gene
