摘要
目的:考察广藿香醇对上皮间质转化(epithelial mesenchymal transition,EMT)及人胃癌细胞株HGC-27侵袭、转移的影响。方法:采用不同浓度广藿香醇体外培养HGC-27细胞株,用MTT法考察广藿香醇对HGC-27细胞增殖的影响;用划痕实验和Transwell实验考察广藿香醇对HGC-27细胞侵袭和迁移的影响;Western印迹法考察广藿香醇对EMT相关蛋白E-cadherin、N-cadherin、β-Catenin、Vimentin表达的影响。结果:广藿香醇能够抑制HGC-27细胞增殖,且呈时间和剂量依赖性,24和48 h的IC50分别为38.9和18.9μmol/L。20和40μmol/L广藿香醇能够显著抑制划痕愈合面积,减少HGC-27细胞迁移和侵袭数量(P<0.05)。20和40μmol/L广藿香醇干预48 h,显著上调E-cadherin蛋白表达,减少N-cadherin、β-Catenin、Vimentin蛋白表达(P<0.05)。结论:广藿香醇可能通过抑制人胃癌细胞HGC-27的EMT而抑制其侵袭、转移能力。
Objective:To investigate the effects of patchouli alcohol on epithelial mesenchymal transition,migration and invasion of human gastric cancer cells HGC-27.Methods:Human gastric cancer cells HGC-27 were cultured in vitro,MTT assay was used to investigate the effect of patchouli alcohol on the growth and proliferation of HGC-27 cells.The effect of patchouli alcohol on the migration and invasion of HGC-27 cells was investigated by scratch and Transwell tests.Western-blot was used to investigate the expression levels of EMT-related proteins(E-cadherin,N-cadherin,β-Catenin,Vimentin).Results:MTT results showed that patchouli alcohol inhibited the proliferation of HGC-27 cells in a time-and dose-dependent manner,and the IC was 38.9μmol/L at 24 h and 18.9μmol/L at 48 h,respectively.20 and 40μmol/Lpatchouli alcohol could significantly inhibit the scratch healing size and reduce the number of migration and invasion of HGC-27 cells(P<0.05).Western-blot showed that the expression of E-cadherin was increased and the expression of N-cadherin,β-Catenin and Vimentin were all decreased(P<0.05)following intervension by 20 and 40μmol/L patchouli alcohol for 48 h.Conclusion:Patchouli alcohol could inhibit the EMT through increasing the expression of E-cadherin and decreasing the expression of N-cadherin,β-Catenin and Vimentin,and inhibit the migration and invasion of HGC-27 cells subsequently.
作者
闵琼
曾海荣
陆翠燕
张夏炎
MIN Qiong;ZENG Hairong;LU Cuiyan;ZHANG Xiayan(Department of Pharmacy,Changhai Hospital,Naval Medical University,Shanghai 200433,China;Department of Pharmacy,Central Hospital of Shanghai Putuo District,Shanghai 200062,China;Department of Pharmacy,Traditional Chinese Medicine Hospital of Shanghai Putuo District,Shanghai 200062,China)
出处
《药学服务与研究》
CAS
2020年第1期6-11,共6页
Pharmaceutical Care and Research
基金
国家自然科学基金(81703880)
关键词
广藿香醇
人胃癌细胞
上皮间质转变
侵袭
转移
patchouli alcohol
human gastric cancer cells
epithelial mesenchymal transition
invasion
metastasis
