摘要
目的探讨全血γ干扰素释放实验在菌阴肺结核和结核性胸膜炎中的诊断价值。方法选择2016年4月至2018年4月来我院治疗的40例初诊肺结核患者以及40例健康对照者作为此次研究对象,分为菌阳肺结核组、菌阴肺结核组、肺部其他疾病对照组以及健康对照组。用ELISA法对上述受试者早期抗原靶(ESAT-6)、ESAT-6/滤过培养蛋白(CFP-10)融合抗原、PPD抗原、刺激后的血浆中γ-干扰素(IFN-γ)的含量进行测定。结果结核组3种抗原刺激的γ干扰素释放实验的阳性率(81.81%)显著高于健康组的阳性率(20.0%),两组间比较具有统计学差异(P<0.05)。菌阳肺结核组患者3种抗原刺激的γ干扰素释放实验与菌阴肺结核组比较无明显差异(P>0.05)。菌阴肺结核组3种抗原刺激的γ干扰素释放实验的阳性率(86.36%)显著高于疾病对照组的阳性率(35.29%),两组间比较具有统计学差异(P<0.05)。结论全血γ释放试验可用于活动性肺结核患者的筛查、菌阴肺结核的诊断和鉴别诊断,值得临床推广应用。
Objective To explore the diagnostic value of total blood radix aconiti interferon release in mycotic tuberculosis and tuberculous pleurisy.Methods 40 patients with newly diagnosed tuberculosis and 40 healthy controls who came to our hospital for treatment from April 2016 to April 2018 were selected as subjects of this study,and were divided into the bacillus positive pulmonary tuberculosis group,the bacillus negative pulmonary tuberculosis group,the control group of other pulmonary diseases and the healthy control group.The concentrations of early antigen target(esat-6),esat-6/filter culture protein(cfp-10),PPD antigen,and plasma y-interferon(ifn-y)were measured by ELISA.Results The positive rate(81.81%)of the 3 antigen-stimulated interferon 1 release in the tuberculosis group was significantly higher than that in the healthy group(20.0%),and there were statistically significant differences between the two groups(P<0.05).There was no significant difference between the 3 antigen-stimulating interferon 1 release test and the bacillus negative pulmonary tuberculosis group(P>0.05).The positive rate(86.36%)of the 1 interferon release test stimulated by 3 antigens in bacillus tuberculosis group was significantly higher than that of the disease control group(35.29%),and there was a statistical difference between the two groups(P<0.05).Conclusion Whole-bloodγrelease test can be used for screening of active pulmonary tuberculosis patients,diagnosis and differential diagnosis of bacillary pulmonary tuberculosis,and is worthy of clinical application.
作者
阳芳
蒋璇
覃超群
高枫
黄斌
YANG Fang;JIANG Xuan;QIN Chaoqun;GAO Feng;HUANG Bin(Guilin people s Hospital of Guangxi Zhuang Autonomous Region,Guilin 541002,China)
出处
《中国老年保健医学》
2020年第1期108-110,共3页
Chinese Journal of Geriatric Care
