摘要
目的:建立参麦袋泡茶的质量控制标准。方法:采用薄层色谱法分别对袋泡茶中的西洋参、菊花进行定性鉴别,并用高效液相色谱法对袋泡茶中的人参皂苷Re、人参皂苷Rb1进行含量测定。色谱条件:色谱柱为Kromasil C18色谱柱(4.6mm×250mm,5μm),流动相为乙腈-水,流速为1.0m L·min-1,柱温为30℃,检测波长为203nm。结果:西洋参、菊花的薄层色谱特征斑点清晰,阴性对照无干扰;人参皂苷Re线性范围为0.800~10.000μg(r=0.9999),平均加样回收率为99.73%,RSD为1.32%;人参皂苷Rb1线性范围为2.216~25.450μg(r=0.9999),平均加样回收率为102.37%,RSD为1.68%。结论:该方法专属性强,重复性好,适用于参麦袋泡茶的质量控制。
Objective:To establish the quality standard of compound Shenmai teabag and to provide the quality assurance.Method:Panacis Quinquefolii Radix and Chrysanthemi flos were identified by thin layer chromatography.HPLC method was used to determine the content of Ginsenoside Re and Ginsenoside Rb1.The determination was performed on a Kromasil C18 column(4.6 mm×250 mm,5μm),with the mobile phase of acetonitrile-water.The flow rate was 1.0m L·min-1,the column temperature was set at 30℃and test wavelength was 203 nm.Result:The TLC spots were clear and well-separated without negative interference.Ginsenoside Re showed good linearity at 0.800~10.000μg(r=0.9999),and the average recovery was 99.73%with RSD 1.32%.Ginsenoside Rb1 showed good linearity at 2.216~25.450μg(r=0.9999),and the average recovery was 102.37%with RSD 1.68%.Conclusion:The process is practical and stable,which could be used for quality control of compound Shenmai teabag.
作者
向云亚
蓝荣
徐晶晶
XIANG Yun-ya;LAN Rong;XU Jing-jing(Xiamen Medical College/Fujian Provincial Key Laboratory of Traditional Chinese Medicine Finish Machining and Health Products Development,Xiamen 361023,China.)
出处
《江西中医药大学学报》
2020年第1期76-79,共4页
Journal of Jiangxi University of Chinese Medicine
基金
厦门医学院科研项目（Z2013-29）
福建省中青年教师教育科研项目（JAT170702）
中药精加工与健康产品开发研究（ZD201405）
关键词
袋泡茶
养阴润肺
薄层色谱
高效液相色谱
质量标准
Teabag
Nourishing Yin and Moistening Lung
Thin Layer Chromatography(TLC)
High Performance Liquid Chromatography(HPLC)
Qualification Standard
