摘要
抽穗期和开花期是衡量小麦发育快慢及稳定性的两个重要时期,发掘相关基因位点并应用于育种选择,可为小麦稳产性改良提供指导。本研究以人工合成六倍体小麦(Turtur)和Triticum spelta L.衍生系(Bubo)为亲本杂交构建的包含186个家系的重组自交系(recom;binant inbred line,RIL)群体(F6)为材料,于2014-2017年连续种植于山东省农业科学院作物研究所济南试验基地,结合已构建的包含5301个标记、长度为2464 c M的高密度遗传连锁图谱对抽穗期和开花期QTL进行定位,结果表明,在1B(3)、2B、4B(2)、5A、5B和7B染色体上共检测到9个抽穗期相关QTL,可解释4.55%~13.40%的表型变异;在1D、2A、3D、4B、5A、6B和7B染色体上共定位到7个开花期相关QTL,可解释3.48%~16.93%的表型变异。其中,7B染色体上4409103~1233594标记区间内控制抽穗期的QTL连续两年被检测到;4B和7B染色体上控制开花期的QTL分别连续2年和3年被检测到。这将为下一步分子标记辅助育种和品种稳产性改良提供理论依据。
Heading time and flowering time are two important traits to measure the growth and stability of wheat. Discovering relevant gene loci and applying them to breeding can provide guidance for wheat yield improvement. In this study,a RIL( recom;binant inbred line) population( F6) consisting of 186 families was constructed by artificially synthesizing hexaploid wheat( Turtur) and T. spelta L. derived line( Bubo) as parents. The materials were continuously planted in Jinan Experimental Base of the Crop Research Institute of Shandong Academy of Agricultural Sciences in 2014-2017. The QTL mapping was carried out at the heading and flowering stages in com;bination with the constructed high-density genetic linkage map containing 5 301 markers and a length of 2 464 cM. The results showed that 9 heading QTLs were mapped on the chromosomes1 B( 3),2 B,4 B( 2),5 A,5 B and 7 B,which explained 4. 55% ~ 13. 40% of phenotypic variation,and 6 flowering QTLs were mapped on the chromosomes 1 D,2 A,3 D,4 B,5 A,6 B and 7 B,explaining 3. 48% ~16. 93% of phenotypic variation. Among them,the QTL of heading time in the 4409103 ~ 1233594 marker interval on chromosome 7 B was detected for 2 consecutive years;the QTL of flowering time on chromosomes 4 B and 7 B were detected in 2 and 3 years respectively. It could provide theoretical references for molecular maker-assisted breeding and stable-yielding improvement.
作者
王克森
董爽爽
李法计
郭军
台述强
王利彬
程敦公
穆平
刘建军
李豪圣
赵振东
曹新有
张玉梅
Wang Kesen;Dong Shuangshuang;Li Faji;Guo Jun;Tai Shuqiang;Wang Libing;Cheng Dungong;Mu Ping;Liu Jianjun;Li Haosheng;Zhao Zhendong;Cao Xinyou;Zhang Yumei(College of Agronomy,Qingdao Agricultural University,Qingdao 266109,China;Crop Research Institute,Shandong Academy of Agricultural Sciences/National Engineering Laboratory for Wheat and Maize/Key Laboratory of Wheat Biology and Genetic Improvement in North Huang-Huai River Valley,Ministry of Agriculture,Jinan 250100,China;Gaomi Plant Quarantine Station,Gaomi 261500,China)
出处
《山东农业科学》
2020年第1期17-23,共7页
Shandong Agricultural Sciences
基金
国家重点研发计划项目(2016YFE0108600,2016YFD0101802)
山东省重大科技创新工程项目(2018YFJH0602)
山东省泰山产业领军人才项目
山东省农业良种工程项目(2019LZGC001)
关键词
小麦
抽穗期
开花期
QTL
Wheat
Heading time
Flowering time
QTL
