摘要
将中华乌塘鳢置于密闭的玻璃容器中,在溶解氧浓度分别为3.5、1.0、0.5、0.3、0.05、0、0 mg/L(将鱼憋2 h后)时分别进行取样,测定肝脏、肠和鳃中的酶活。结果表明,中华乌塘鳢肝脏组织中的抗氧化酶活性最高,肠组织中免疫酶活性最高,而外周组织(鳃)的酶活性远低于其他组织;当溶解氧浓度降至约0.31 mg/L时,抗氧化酶和免疫酶活性均明显下降,这可能是中华乌塘鳢进入了一种类似休眠的状态,整个新陈代谢降低仅能维持基本的生命状态;当水中溶解氧几乎消耗完全时,中华乌塘鳢为维持其基本生命特征而进行自身调节,抗氧化酶和免疫酶的活性又出现明显升高。
Chinese black sleepers were cultured in closed glass vessels,and samples were collected when the concentration of dissolved oxygen was 3.5,1.0,0.5,0.3,0.05,0 and 0 mg/L(dissolved oxygen disappeared for 2 hours).The enzyme activities in the liver,intestine and gill were determined.The results showed that the activity of antioxidant enzymes was the highest in the liver and the activity of immune enzymes were most active in intestinal tissues,while both of them were much lower in the gill than that in the liver and intestinal tissues.When the dissolved oxygen concentration dropped to 0.31 mg/L,the activities of antioxidant enzymes and immune enzymes decreased significantly,this might be caused by the fact that Chinese black sleepers entered a state similar to dormancy,so that the entire metabolism was reduced only to maintain the basic state of life.However,when dissolved oxygen in water was almost consumed completely,the activities of antioxidant enzymes and immune enzymes increased again,which might be derived from the self-regulation of Chinese black sleepers to maintain its basic life characteristics.
作者
马粒雅
王闻
迟雯丹
王晓
王玲
郑明刚
范士亮
MA Li-ya;WANG Wen;CHI Wen-dan(College of Environmental Science and Engineering,Qingdao University,Qingdao,Shandong 266071;The First Institute of Oceanography,Ministry of Natural Resources,Qingdao,Shandong 266061)
出处
《安徽农业科学》
CAS
2020年第3期91-94,共4页
Journal of Anhui Agricultural Sciences
基金
海洋公益性行业科研专项经费项目(201505028-4)
中央级公益性科研院所基本科研业务费专项(2016Q07)
关键词
中华乌塘鳢
溶解氧
碱性磷酸酶
超氧化歧化酶
过氧化氢酶
Chinese black sleeper
Dissolved oxygen
Alkaline phosphatase(AKP)
Superoxide dismutase(SOD)
Catalase(CAT)
