摘要
目的研究神经丝蛋白轻链多肽(NEFL)在I型神经纤维瘤病变(NF1)组织中的表达及意义。方法回顾性分析2017年1月~2019年6月本院收治的I型神经纤维瘤患者34例为研究对象,收集手术或病理活检切除的神经纤维瘤瘤变组织,另取因整容或其他皮肤疾病手术患者术中切除的正常皮肤组织34例为对照组,采用荧光定量PCR(RT-qPCR)检测NF1及正常皮肤组织中NEFL mRNA、结缔组织生长因子(CTGF)mRNA及其下游效应分子转化生长因子β1(TGF-β1)mRNA表达水平,采用免疫印迹(WB)检测NEFL、CTGF、TGF-β1蛋白表达水平,采用Pearson相关性分析NF1组织中NEFL蛋白水平与CTGF、TGF-β1蛋白的相关性。结果与正常皮肤组织比较,NF1瘤变组织中NEFL、CTGF、TGF-β1 mRNA及蛋白水平均显著增加(P<0.05)。Pearon法分析结果显示,NF1瘤变组织中NEFL蛋白水平与CTGF、TGF-β1蛋白水平均正相关(r=0.471、0.610,P<0.05)。结论NEFL在I型神经纤维瘤组织中高表达,可能通过组织纤维化参与NF1发生发展。
Objective To study the expression and significance of neurofilament protein light chain polypeptide(NEFL)in neurofibromatosis type I(NF1).Methods 34 patients with neurofibroma type I admitted to our hospital from January 2017 to June 2019 were retrospectively analyzed.Neurofibroma transformed tissues excised by surgical or pathological biopsy were collected,and 34 normal skin tissues excised during surgery for cosmetic or other skin diseases were taken as control group.The expression levels of NEFL mRNA,connective tissue growth factor(CTGF)mRNA and its downstream effector molecule transforming growth factor-β1(TGF-β1)mRNA in NF1 and normal skin tissues were detected by fluorescence quantitative PCR(RT-qPCR),the expression levels of NEFL,CTGF and TGF-β1 proteins were detected by immunoblotting(WB),Pearson correlation was used to analyze the correlations between NEFL protein level and CTGF,TGF-β1 proteins in NF1 tissue.Results Compared with normal skin tissue,the levels of NEFL,CTGF,TGF-β1 mRNAs and proteins in NF1 tumorigenic tissue were significantly increased(P<0.05).Pearon analysis showed that NEFL protein level in NF1 tumorigenic tissues were positively correlated with CTGF and TGF-β1 protein levels(r=0.471,0.610,P<0.05).Conclusions NEFL is highly expressed in neurofibroma type I and may be involved in the development of NF1 through tissue fibrosis.
作者
牛学强
刘洋
刘福云
胡伟明
NIU Xueqiang;LIU Yang;LIU Fuyun(Department of Pediatric Orthopaedics,The Third Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China)
出处
《中风与神经疾病杂志》
CAS
2019年第11期1013-1016,共4页
Journal of Apoplexy and Nervous Diseases
基金
2016年河南省医学科技攻关计划(No.201602134)
关键词
神经纤维瘤
神经丝蛋白轻链多肽
表达
意义
Neurofibromatosis
Neurofilament protein light-chain polypeptide
Expression
Significance
