摘要
目的分析辛伐他汀纳米粒对脓毒症致急性肺损伤(ALI)小鼠肺组织诱导型一氧化氮合酶(i NOS)/内皮型一氧化氮合酶(e NOS)平衡及预后的影响。方法取45只健康雄性C57/BL6小鼠,随机分为假手术组(开腹,但不结扎穿孔)、模型组(经盲肠结扎穿孔术制备脓毒症小鼠模型)和干预组(脓毒症小鼠模型+尾静脉注射辛伐他汀纳米粒制剂),每组各15只。造模后24 h,通过苏木精-伊红染色观察三组小鼠病理形态学改变,采用免疫组织化学法测定三组小鼠肺组织中i NOS和e NOS表达水平,记录小鼠造模1周时的生存状况。结果经苏木精-伊红染色结果发现,假手术组小鼠肺组织无明显病理改变;模型组小鼠肺组织细胞排卵紊乱、局部肺组织完整性受破坏、肺泡间中隔增厚、肺泡腔缩小、肺间质弥漫性水肿、弥漫性中性粒细胞浸润;相比模型组,干预组小鼠肺组织中性粒细胞渗出明显减少、肺组织损伤程度较轻、肺泡完整性较好。模型组肺组织中i NOS表达水平明显高于假手术组,干预组i NOS表达水平明显低于模型组(P<0.01);模型组肺组织中e NOS表达水平明显低于假手术组,干预组e NOS表达水平明显高于模型组(P<0.01)。模型组造模1周时的生存率(6.67%)低于假手术组(93.33%),干预组生存率(66.67%)高于模型组,差异均有显著性(P<0.05)。结论辛伐他汀纳米粒可保护脓毒症致ALI小鼠肺组织损伤,调节i NOS/e NOS平衡,可能是脓毒症致ALI具有保护效应的潜在处理位点。
Objective To analyze the effect of simvastatin nanoparticles on the balance of inducible nitric oxide synthase( i NOS)/endothelial nitric oxide synthase( e NOS) in lung tissue of sepsis-induced acute lung injury( ALI) mice and its prognosis. Methods Forty-five healthy male C57/BL6 mice were randomly divided into sham operation group( open but not ligated perforation),model group( sepsis mice model prepared by cecal ligation and perforation) and intervention group( sepsis mice model + tail vein injection of simvastatin nanoparticles preparation),15 mice in each group. 24 hours after modeling,the pathological changes of three groups of mice were observed by hematoxylin-eosin staining. The expression levels of i NOS and e NOS in lung tissues of three groups of mice were measured by immunohistochemical method,and the survival status of mice at one week after modeling was recorded. Results The results of hematoxylin-eosin staining showed that there were no obvious pathological changes in lung tissues in sham-operated mice. In model mice,ovulation disorder,destruction of local lung tissue integrity,thickening of alveolar septum,shrinkage of alveolar cavity,diffuse pulmonary interstitial edema and diffuse neutrophil infiltration were found in model group. In the intervention group,neutrophil exudation in lung tissue was significantly reduced,the degree of lung injury was mild,and alveolar integrity was better. The expression level of i NOS in lung tissue of model group was significantly higher than that of sham-operated group.The expression level of i NOS in intervention group was significantly lower than that of model group( P < 0. 01). The expression level of e NOS in lung tissue of model group was significantly lower than that of sham-operated group,and the expression level of e NOS in intervention group was significantly higher than that of model group( P < 0. 01). The survival rate of model group at 1 week was lower than that of sham operation group,and that of intervention group was higher than that of model group
作者
胡萍
胡倩
张秀霞
HU Ping;HU Qian;ZHANG Xiu-xia(Department of Emergency Medicine,Xi'an No.4 Hospital in Shaanxi Province,Xi'an Shaanxi 710004,China;Zhengzhou Railway Vocational and Technical College,Zhengzhou Henan 451460,China;General Medicine,Sanmenxia Elderly Care Hospital,Sanmenxia Henan 472000,China)
出处
《临床和实验医学杂志》
2020年第4期341-344,共4页
Journal of Clinical and Experimental Medicine
基金
陕西省中医管理局中医药科研课题(编号:JCPT044)
