摘要
【目的】观察天麻舒心方对自发性高血压大鼠(SHR)动脉管壁形态及血浆和肠系膜动脉组织中血管紧张素Ⅱ(AngⅡ)、血管紧张素1-7[Ang(1-7)]含量的影响。【方法】将75只SHR大鼠随机分为模型对照组,西药对照组,中药高、中、低剂量组,每组15只;另将16只Wistar大鼠设为正常对照组。中药高、中、低剂量组给予天麻舒心浸膏液(分别对应剂量为11.16、5.58、2.79 g·kg^-1·d^-1)灌胃;西药对照组给予氯沙坦钾混悬液(剂量为17.5 mg·kg^-1·d^-1)灌胃;模型对照组及正常对照组给予同体积蒸馏水灌胃。共18周。期间给予动态血压监测。给药结束后,采用苏木素-伊红(HE)染色观察肠系膜动脉形态学变化,125I放射免疫分析法测定血浆及血管组织的AngⅡ、Ang(1-7)水平。【结果】给药前,模型对照组血压水平高于正常对照组(P<0.01);给药2周后,中药高、中剂量组及西药对照组血压均显著低于模型组(P<0.01),给药14周后,各中药组及西药对照组血压均显著低于模型组(P<0.01)。SHR肠系膜动脉形态学明显改变;而各中药组及西药对照组可明显改善SHR肠系膜动脉形态学变化。与正常对照组比较,模型对照组血浆和血管AngⅡ水平显著增高(P<0.01),血浆和血管Ang(1-7)水平均显著降低(P<0.05或P<0.01);与模型对照组比较,各中药组及西药对照组血浆及血管局部AngⅡ水平均无显著差异(P>0.05),中药高、中剂量组血浆Ang(1-7)显著升高(P<0.01),各中药组及西药对照组血管局部Ang(1-7)均显著升高(P<0.05或P<0.01)。【结论】SHR大鼠存在血管重塑。组织局部AngⅡ增高可能是高血压阻力血管重塑的可能机制之一。天麻舒心方可能通过增加整体及阻力血管局部Ang(1-7)发挥降低血压、改善SHR阻力血管富营养重塑的作用。
Objective To observe the effect of Tianma Shuxin Recipe on the arterial wall morphology and contents of angiotensin(Ang)Ⅱand Ang(1-7)in plasma and mesenteric artery of spontaneously hypertensive rats(SHR).Methods Seventy-five SHR were randomly divided into model control group,western medicine control group,and high-,middle-and low-dose Chinese medicine groups,15 rats in each group.Additionally,16 Wistar rats were set up as the normal control group.The high-,middle-and low-dosage Chinese medicine groups were given intragastric administration of Tianma Shuxin Recipe(at the dosage of 11.16,5.58,2.79 g·kg^-1·d^-1,respectively),the western medicine control group was given intragastric administration of losartan potassium suspension(at the dosage of 17.5 mg·kg^-1·d^-1),and the model control group and normal control group were given intragastric administration of the same volume of distilled water.The treatment lasted 18 days.During the period,the ambulatory blood pressure monitoring was carried out.After the treatment,the morphological changes of the mesenteric artery were observed by hematoxylin-eosin(HE)staining,and the levels of AngⅡand Ang(1-7)in plasma and vascular tissues were determined by125 I radioimmunoassay.Results Before treatment,the blood pressure in the model control group was higher than that in the normal control group(P<0.01).After 2-week treatment,the blood pressure in the high-and middle-dose Chinese medicine groups and western medicine control group was lower than that in the model control group(P<0.01),and after 14-week treatment,the blood pressure in the three Chinese medicine groups and western medicine control group was lower than that in the model control group(P<0.01).The morphological changes of the SHR mesenteric artery were seen,while those changes were improved in the three Chinese medicine groups and western medicine control group.Compared with the normal control group,the levels of AngⅡin plasm and vessels of the model control group were increased(P<0.01),and the levels of Ang
作者
刘坤
徐贵成
王卉
单雅蒙
潘立敏
王洋
LIU Kun;XU Gui-Cheng;WANG Hui;SHAN Ya-Meng;PAN Li-Min;WANG Yang(Guang'an Men Hospital,China Academy of Chinese Medical Sciences,Beijing 100053,China)
出处
《广州中医药大学学报》
CAS
2020年第1期115-120,共6页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
国家自然科学基金面上项目（编号：81173261）
关键词
天麻舒心方
高血压
血管重塑
血管紧张素
疾病模型
动物
大鼠
Tianma Shuxin Recipe
hypertension
vascular remodeling
angiotensin
disease models,animal
rats
