摘要
目的:探讨药物诱导联合腹主动脉流出道缩窄构建兔腹主动脉瘤(AAA)模型的可行性与有效性。方法:将24只雌性新西兰大白兔随机均分为4组,其中两组分别采用含CaCl2(0.75 mol/L)或胰蛋白酶(0.04 g/m L)溶液的棉条包裹浸润血管30 min诱导AAA,另两组分别在CaCl2或胰蛋白酶浸润的基础上行腹主动脉流出道缩窄术(缩窄50%~60%)造模。术后使用兽用彩超诊断仪监测受累血管管径变化,术后2周,收集损伤段腹主动脉制作组织切片行HE与EVG染色。用计算机模拟评估流出道缩窄对AAA形成的影响。结果:术后2周,超声检查显示,两个单纯药物浸润组受累血管扩张不明显,均未达到AAA形成标准,两个药物联合缩窄组受累血管明显扩张,其中CaCl2+缩窄组成瘤率66.67%(4/6),血管平均扩张1.61倍;胰蛋白酶+缩窄组成瘤率83.33%(5/6),血管平均扩张1.89倍。与正常腹主动脉比较,CaCl2浸润的血管内膜厚度明显增加(均P<0.05),而胰蛋白酶浸润后的血管内膜厚度变化不明显(均P>0.05);各组中膜厚度均明显增加,弹力纤维面积百分比均明显降低,其中CaCl2+缩窄组的变化最为明显(均P<0.05)。计算机数值模拟结果显示,流出道缩窄后血管壁应力增大,成瘤率增加。结论:药物损伤联合腹主动脉流出道缩窄能成功构建兔AAA模型,缩短造模时间,且胰蛋白酶浸润损伤联合腹主动脉流出道缩窄造模方法优于CaCl2联合腹主动脉流出道缩窄造模方法。
Objective: To investigate the feasibility and effectiveness of creating rabbit abdominal aortic aneurysm(AAA) model by using drug induction plus abdominal aortic outflow coarctation.Methods: Twenty-four female New Zealand white rabbits were equally randomized into 4 groups, in which,AAA model was induced by wrapping and infiltrating the vessel with a cotton strip containing a solution of CaCl2(0.75 mol/L) or trypsin(0.04 g/m L) in two groups, and the model was constructed by CaCl2 or trypsin infiltration plus abdominal aorta outflow constriction(50% 60% constriction) in the other two groups. After the operation, the change in diameter of the affected blood vessel was monitored by veterinary ultrasound. The experimental animals were sacrificed 2 weeks after the operation, and the injured abdominal aorta was harvested to prepare tissue sections for HE and EVG staining. The effect of outflow tract coarctation on AAA formation was evaluated by computer simulation.Results: Ultrasound examination on 2 weeks after operation showed that no evident dilatation of the affected vessel was seen, and no vessel reached the standard of AAA formation in the two groups undergoing drug infiltration alone;the affected vessel was obviously dilated in the two group undergoing drug infiltration plus abdominal aortic outflow coarctation, in which, the AAA formation rate was 66.67 %(4/6) in CaCl2 plus coarctation group, with an average 1.61-fold expansion, and the AAA formation rate was 83.33%(5/6) in trypsin plus coarctation group, with an average 1.89-fold expansion. Compared with the normal abdominal aorta, the thickness of abdominal aorta intima was significantly increased after CaCl2 infiltration(both P<0.05), but the thickness of abdominal aorta intima did not significantly change after trypsin infiltration(both P>0.05);the thickness of the tunica media was significantly increased and the percentage of the area occupied by the elastic fibers was significantly reduced in all groups, and these changes were most evident in CaCl2 plus
作者
赵梦婕
任玥颖
胡璇
孙安强
陈增胜
徐在品
ZHAO Mengjie;REN Yueying;HU Xuan;SUN Anqiang;CHEN Zengsheng;XU Zaipin(College of Animal Science,Guizhou University,Guiyang 550025,China;School of Biological and Medical Engineering,Beihang University,Beijing 100083,China;Biomedical Engineering High-tech Innovation Center,Beihang University,Beijing 100083,China)
出处
《中国普通外科杂志》
CAS
CSCD
北大核心
2019年第12期1482-1489,共8页
China Journal of General Surgery
基金
国家自然科学基金资助项目（11862004
11872096）
黔科合平台人才基金资助项目（[2018]5781）
关键词
主动脉瘤
腹
模型
动物
兔
Aortic Aneurysm, Abdominal
Models, Animal
Rabbits
