摘要
目的:研究肉苁蓉总苷在人工胃、肠液中的代谢转化,推测其在体内的代谢转化途径。方法:采用超高效液相色谱串联飞行时间质谱法。色谱柱为ACQUITY UPLC BEH,柱温为25℃,流动相为0.2%甲酸水-乙腈溶液(梯度洗脱),流速为0.2 mL/min,检测波长为330 nm;离子源为电喷雾离子源,质荷比(m/z)为50→1 000;在正、负离子模式下,对肉苁蓉总苷在人工胃、肠液中的代谢成分进行鉴定分析,并结合文献推测肉苁蓉总苷在人工胃、肠液中代谢途径。结果:肉苁蓉总苷经人工胃液代谢后,共推测出69个成分,其中14个原型成分(如芥子醛葡萄糖苷、胡萝卜苷等)、55个代谢成分(如Methyl-O-Kankanoside J、Methyl-O-Kankanoside E等),推测其代谢途径有甲基化、去甲基化、羟基化、甲氧基化、乙酰化、硫酸化、葡萄糖醛酸化。肉苁蓉总苷经人工肠液代谢后,共推测出90个成分,其中4个原型成分(如Kankanoside M、Kankanoside L等)、86个代谢成分(如Methyl-O-Kankanoside、Methyl-O-Kankanoside E等),推测其代谢途径有甲基化、去甲基化、羟基化、去羟基化、甲氧基化、乙酰化、硫酸化、葡萄糖醛酸化。结论:本研究初步推测肉苁蓉总苷在人工胃、肠液中可能通过甲基化、去甲基化、羟基化等多种途径进行代谢转化,可为肉苁蓉总苷的体内代谢转化研究提供参考依据。
OBJECTIVE:To study the metabolic transformation of total glycosides of Cistanche deserticola in artificial gastric and intestinal juice,and to speculate its metabolic transformation pathway in vivo. METHODS:UPLC/Q-TOF-MS was adopted.The determination was performed on ACQUITY UPLC BEH column with mobile phase consisted of 0.2% formic acid wateracetonitrile(gradient elution)at the flow rate of 0.2 mL/min. The detection wavelength was set at 330 nm,and column temperature was 25 ℃. The ion source was electrospray ion source,and mass to charge ratio(m/z)was 50→1 000. In the positive and negative ion mode,the metabolic components of the total glycosides of C. deserticola in artificial gastric and intestinal juice were identified analysis,and combined with the literature,the metabolic pathway of total glycosides of C. deserticola in artificial gastric and intestinal juice was speculated. RESULTS:After the total glycosides of C. deserticola were metabolized by artificial gastric juice,and a total of 69 components were estimated, including 14 prototype components(such as Mustard aldehyde glucoside,daucosstorol)and 55 metabolic components(such as Methyl-O-Kankanoside J,Methyl-O-Kankanoside E),it is speculated that its metabolic pathways were methylation,demethylation,hydroxylation,methoxylation,acetylation,sulfation,and glucuronidation.After the total glycosides of C. deserticola were metabolized by artificial intestinal juice,a total of 90 components were estimated,including 4 prototype components(such as Kankanoside M,Kankanoside L) and 86 metabolic components(such as Methyl-OKankanoside, Methyl-O-Kankanoside E). It was speculated that its metabolic pathways were methylation, demethylation,hydroxylation, dehydroxylation, methoxylation, acetylation, sulfation and glucuronidation. CONCLUSIONS: This study preliminarily speculates that the total glycosides of C. deserticola may be metabolized by methylation, demethylation,hydroxylation and other metabolic pathway in artificial gastrointestinal juice,which may provide ref
作者
王昕蕾
刘博男
张蒙蒙
刘贵玉
胡扬
孙向明
李文兰
WANG Xinlei;LIU Bonan;ZHANG Mengmeng;LIU Guiyu;HU Yang;SUN Xiangming;LI Wenlan(College of Pharmacy,Harbin University of Commerce,Harbin 150076,China)
出处
《中国药房》
CAS
北大核心
2020年第1期53-61,共9页
China Pharmacy
基金
黑龙江省科学基金项目(No.ZD2017014)
哈尔滨商业大学校科学基金项目(No.18XN083)
关键词
肉苁蓉总苷
代谢
人工胃液
人工肠液
超高效液相色谱串联飞行时间质谱法
成分分析
Total glycosides of Cistanche deserticola
Metabolism
Artificial gastric juice
Artificial intestinal juice
UPLC/QTOF-MS
Component analysis
