摘要
目的:探究苦参碱联合化疗药物对人急性髓系白血病HL-60细胞增殖及侵袭力的影响。方法:体外培养人急性髓系白血病HL-60细胞,苦参碱单药组以1.0 g/L苦参碱干预,三氧化二砷单药组以3μmol/L三氧化二砷处理,联合组以终浓度为1.0 g/L的苦参碱+3μmol/L三氧化二砷处理,空白组以等量生理盐水处理。MTT法检测HL-60细胞增殖情况,ranswell小室实验检测HL-60侵袭力,蛋白免疫印迹法检测HL-60细胞MMPs蛋白表达情况。结果:苦参碱单药组、三氧化二砷单药组、联合组24 h PIR分别为(18.54±2.54)%,(29.54±2.62)%,(41.45±3.72)%,48 h PIR分别为(27.46±2.55)%,(38.93±3.76)%,(45.64±3.54)%,3组内HL-60的PIR均随着培养时间的增加而增加,其中联合组24 h、48 h PIR均高于同时间点的苦参碱单药组、三氧化二砷单药组,差异有统计学意义(P<0.05)。空白组、苦参碱单药组、三氧化二砷单药组、联合组侵袭细胞分别为(127.64±23.93)个、(94.24±15.23)个、(81.23±12.21)个、(75.13±10.69)个,相对侵袭指数分别为(100.00±0.00)%、(73.44±0.34)%、(62.47±0.15)%、(58.54±0.36)%;MMP-2蛋白相对表达量分别为(0.91±0.05)、(0.39±0.04)、(0.23±0.04)、(0.14±0.03);MMP-9蛋白相对表达量分别为(0.62±0.05)、(0.31±0.05)、(0.27±0.04)、(0.16±0.02)。与空白组比较,苦参碱单药组、三氧化二砷单药组、联合组侵袭细胞数量显著减少,相对侵袭指数及细胞中MMP-2、MMP-9相对表达量降低,且联合组均低于苦参碱单药组、三氧化二砷单药组,差异有统计学意义(P<0.05)。结论:苦参碱联合三氧化二砷通过下调MMP-2、MMP-9蛋白表达有效抑制人急性髓系白血病HL-60细胞增殖及侵袭。
Objective:To investigate the effects of matrine combined with chemotherapeutic drugs on the proliferation and invasion of human acute myeloid leukemia HL-60 cells.Methods:Human acute myeloid leukemia HL-60 cells were cultured in vitro.Matrine monotherapy group was treated with 1.0 g/L matrine,arsenic trioxide monotherapy group with 3 mmol/L arsenic trioxide,combination group with 1.0 g/L matrine+3 mmol/L arsenic trioxide,blank group with the same amount of saline.MTT assay was used to detect the proliferation of HL-60 cells,ranswell chamber assay was used to detect the invasion ability of HL-60 cells,and Western blotting was used to detect the expression of MMPs in HL-60 cells.Results:The 24-hour PIR of matrine group,arsenic trioxide group and combination group were(18.54±2.54)%,(29.54±2.62)%,(41.45±3.72)%and 48-hour PIR were(27.46±2.55)%,(38.93±3.76)%,(45.64±3.54)%.The PIR of HL-60 in the 3 groups increased with the increase of culture time.PIR of 24 h and 48 h in THE combination group was higher than that of matrine and arsenic trioxide at the same time point(P<0.05).The number of invasive cells in the blank group,the matrine group,the arsenic trioxide group and the combination group were(127.64±23.93),(94.24±15.23),(81.23±12.21),(75.13±10.69),and the relative invasion index were(100.00±0.00),(73.44±0.34),(62.47±0.15),(58.54±0.36)%,respectively.The relative expressions of MMP-2 were(0.91±0.05),(0.39±0.04),(0.23±0.04),(0.14±0.03),and the MMP-9 protein were(0.62±0.05),(0.31±0.05),(0.27±0.04),(0.16±0.02),respectively.Compared with the blank group,the number of invasive cells in matrine monotherapy group,arsenic trioxide monotherapy group and combination group decreased significantly,the relative invasion index and the relative expression of MMP-2 and MMP-9 protein in cells decreased,and the combined group was lower than matrine monotherapy group and arsenic trioxide monotherapy group(P<0.05).Conclusion:Matrine combined with arsenic trioxide can effectively inhibit the proliferation and invasi
作者
晁荣
胡晓燕
朱生东
邓伟
王莉
Chao Rong;Hu Xiaoyan;Zhu Shengdong;Deng Wei;Wang Li(Department of Pediatric Comprehensive Internal Medicine,Gansu Maternal and Child Health Hospital,Lanzhou 730050,China)
出处
《世界中医药》
CAS
2019年第11期2910-2913,共4页
World Chinese Medicine
基金
甘肃省自然科学基金项目(2018-0405-JCC-0846)
