摘要
目的:鉴定从重庆云阳橘红基地采集到疑似橘红褐斑病样品病原菌。方法:使用组织分离、形态学鉴定、真菌ITS序列分析及致病性测定。结果:将其鉴定为橘红褐斑病,病原菌为交链格胞菌(Alternaria alternata)。生物学特性分析表明,该病原菌菌丝生长适宜温度为15~30℃,最适温度为20~25℃,37℃时不能生长;pH值为4~12时均可生长,最适pH值为10;光照对菌丝生长影响不大;该菌可利用多种碳、氮源,最适碳源为果糖和D-甘露醇,最适氮源为蛋白胨和硝酸钠。结论:不同褐斑病菌株间存在一定的差异。
Objective:To identify the pathogens suspected of orange-red brown spot samples collected from the orange-red base in Yunyang,Chongqing.Methods:Tissue isolation,morphological identification,sequence analysis of fungal ITS and pathogenicity were used.Results:It was identified as orange-red brown spot,and the pathogen was Alternaria alternata.The analysis of biological characteristics showed that the suitable temperature for the growth of the pathogenic mycelia was 15~30℃,the optimal temperature was 20~25℃,and it cannot grow at 37℃;it can grow at pH 4~12,and the optimal pH value was 10;Light had little effect on the growth of mycelium;the bacteria can use a variety of carbon and nitrogen sources,the most suitable carbon sources were fructose and D-mannitol,and the most suitable nitrogen sources were peptone and sodium nitrate.Conclusion:There are some differences between different brown spot strains.
作者
杜用玺
徐扬
吕朝耕
池秀莲
王升
康传志
孙楷
袁杰
王铁霖
郭兰萍
Du Yongxi;Xu Yang;Lyu Chaogeng;Chi Xiulian;Wang Sheng;Kang Chuanzhi;Sui Kai;Yuan Jie;Wang Tielin;Guo Lanping(National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences,State Key Laboratory Breeding Base of Dao-di Herbs,Beijing 100700,China;School of Traditional Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou 510006,China)
出处
《世界中医药》
CAS
2019年第11期2845-2848,2854,共5页
World Chinese Medicine
基金
国家重点研发计划项目(2017YFC1700701)
财政部中央本级专项(2060302)
国家发改委标准化项目(ZYBZH-C-HLJ-17,ZYBZH-C-GD-07)
现代农业产业技术项目(CARS-21)
中央级公益性科研院所基本科研业务费专项资金资助项目(ZZXT201806)
中央本级重大增减支项目“名贵中药资源可持续利用能力建设”(2060302)
关键词
橘红褐斑病菌
病原鉴定
生物学特性
Alternaria alternate
Pathogen identification
Biological characterization
