摘要
利用16S rDNA测序方法研究大鼠灌胃玫瑰水提物对肠道微生物的影响,结合转录组测序结果探讨玫瑰水提物对机体的功能作用。选用6周龄SD大鼠,灌胃蒸馏水作为对照组,实验组分别灌胃低、中、高剂量玫瑰水提物,每组8只。结果表明:低、中、高剂量玫瑰水提物均对正常大鼠血脂和生长发育指标无显著影响;不同组别的大鼠粪便中普雷沃氏菌科(Prevotellaceae)和盲肠内容物中普雷沃氏菌属(Prevotella)含量差异明显,均为高剂量组<对照组<中剂量组<低剂量组;与对照组相比,不同剂量的玫瑰水提物均显著影响大鼠肝脏的转录组学,低剂量玫瑰水提物有101个差异表达基因,显著富集于抗原加工和提呈(ko04612)通路;中剂量组也有101个差异表达基因,主要集中在抗原加工和提呈、TNF信号通路(ko04668)等4条通路高剂量组有76个差异表达基因,主要集中在抗原加工和提呈、MAPK信号通路(ko04010)等6条通路不同剂量之间也有明显变化。这说明玫瑰水提物对大鼠肠道普雷沃氏菌属影响较大,同时也显著影响肝脏免疫应答。提示玫瑰水提物可通过干预肠道微生物多样性进而影响机体健康,并具有潜在的诱导肿瘤细胞衰老和提高机体免疫功能的作用。
The method of 16S rDNA sequencing was used to explore effect of rose-flower water extract(RWE)on intestinal flora in rats,and combining with the results of transcriptomic analysis to explore the role of the function of RWE.SD rats full of 6-week-age were sacrificed and controlled group(ZC)were gavaged with distilled water,experimental group were respectively gavaged with low,medium and high-dose of RWE,each group with 8 rats.The results indicated that low,medium and high-dose of RWE had no significant effect on index of blood lipid and index of growth and development of normal rats.The difference in the content of Prevotellaceae in rats’faeces and the content of Prevotella in rats’cecum were obvious.They were all high-dose group<controlled group<medium-dose group<low-dose group.Compared with controlled group,the liver transcriptome of rats was significantly affected by different dose of RWE.The differentially expressed genes of low-dose of RWE was 101,and the significantly enrichment pathway was antigen processing and presentation(ko04612).The differentially expressed genes of medium-dose of RWE was also 101,and they mainly enriched in 4 pathways including the pathway of antigen processing and presentation and TNF signaling pathway(ko04668),etc.The differentially expressed genes of high-dose of RWE was 76,and they mainly enriched in 6 pathways including antigen processing and presentation and MAPK signaling pathway(ko04010),etc.There were also significant changes in the doses.The research indicated that RWE had a significant influence on Prevotella on intestinal tract of rat and also significantly affected the immune response of the liver.It also suggested that RWE can affect the body's health by interfering with the diversity of intestinal microbial and it had the potential to induce the aging of tumor cells and improve the immune function of the body.
作者
周小飞
熊文艳
吴恩凯
张欢
张婷婷
彭春秀
Zhou Xiaofei;Xiong Wenyan;Wu Enkai;Zhang Huan;Zhang Tingting;Peng Chunxiu(College of Food Science and Technology,Yunnan Agricultural University,Kunming 650201;College of Horticulture and Landscape,Yunnan Agricultural University,Kunming 650201)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2020年第1期10-19,共10页
Journal of Chinese Institute Of Food Science and Technology
基金
云南省教育厅科学研究基金资助性项目（2016zzx102）
云南省大创项目（2016106760024）
