摘要
【目的】瓜实蝇是世界性检疫害虫,被列入我国口岸常年监测计划中。本研究以检疫性有害生物瓜实蝇为研究对象,利用不同地理种群瓜实蝇的转录组发现特异性的微卫星(simple sequence repeats,SSR)序列,分析我国口岸监测的瓜实蝇样本的遗传多样性。【方法】利用生物信息学方法,对4个不同地理来源的瓜实蝇转录组进行分析,发现了特异性的SSR序列,并设计特异性引物,选用我国11个省市的49个瓜实蝇监测样本进行测试及验证,使用NTsys和Popgene 32软件进行遗传多样性分析。【结果】筛选出15对多态性较好的引物,UPGMA聚类分析显示,新疆、四川和广西种群归为一支,浙江、广东、江苏种群归为一支,上海、云南、海南、天津种群归为一支,北京种群单独聚为一支。【结论】聚类分析结果显示,11个不同省市的瓜实蝇监测样本间基因分化系数为0.6712,表明不同口岸的瓜实蝇监测样本间的遗传分化较大,可能具有不同的来源地,可为进一步开发监测样本溯源技术提供理论依据。
【Aim】Understanding the spread routes and the origin of quarantine pests is important in international trade.Bactrocera cucurbitae is a worldwide quarantine pest and is also included in the Chinese quarantine list.We aimed to study the genetic differences of B.cucurbitae populations intercetped at different customs ports in China by simple sequence repeats(SSR)identified from transcriptome data.【Method】Polymorphic primers were designed based on the SSR obtained from four transcriptomes of B.cucurbitae in different regions by bioinformatics methods.Forty-nine B.cucurbitae samples from eleven provinces/regions of China were selected for verification,and the NTsys and Popgene 32 softwares were used to analyze their genetic diversity.【Result】Fifteen pairs of primers with reliable polymorphism were selected.An UPGMA cluster analysis clustered the populations of Xinjiang,Sichuan and Guangxi into one clade,the populations of Zhejiang clustered with the ones of Guangdong and Jiangsu,and the populations of Shanghai clustered with the ones of Yunnan,Hainan;the populations from Beijing formed a separate cluster.【Conclusion】The genetic differentiation coefficient among populations was 0.6712,indicating that B.cucurbitae populations in different regions have varied genetic background,and these populations might have different original sources.These results provide the theory and technique support to identify the origins of quarantine pests.
作者
许佳丹
王书平
蒋费涛
朱雅君
叶军
李飞
XU Jiadan;WANG Shuping;JIANG Feitao;ZHU Yajun;YE Jun;LI Fei(Technical Center for Animal Plant and Food Inspection and Quarantine,Shanghai Customs,Shanghai 201708,China;Institute of Insect Sciences,Zhejiang University,Hangzhou,Zhejiang 310058,China;School of Environmental and Chemical Engineering,Chongqing Three Gorges University,Chongqing 404000,China)
出处
《生物安全学报》
CSCD
2019年第4期259-268,共10页
Journal of biosafety
基金
国家重点研发计划项目(2017YFC1200602)
上海出入境检验检疫局科技项目(HK16-010)
关键词
瓜实蝇
转录组
微卫星标记
遗传多样性
Bactrocera cucurbitae
transcriptome
microsatellite marker
genetic diversity
