摘要
采用流式细胞术,对2个芋[Colocasiaesculenta(L.)Schott]品种苏芋1号和香蕉芋的倍性水平和DNA含量进行了测定,2倍基因组DNA含量分别为7.71 pg和5.25 pg,倍性分别为三倍体和二倍体。同时通过HiSeq 2500测序,分别获得240.53 Gb和87.69 Gb数据,芋基因组大小为2.08~2.38 Gb,GC含量为42.57%~42.75%,杂合度为0.52%~2.29%。根据苏芋1号的基因组数据,组装得到基因组序列为2.27 Gb,Contig N50为359 bp;共注释获得24302个基因,99.43%的基因可注释到6个公共数据库;筛选出928987个SSR位点。
In this study,the ploidy levels and DNA contents of two Colocasia esculenta varieties,Suyu No.1 and Xiangjiaoyu from China,were determined by flow cytometry(FCM).The 2C DNA contents of them were 7.71 pg and 5.25 pg,respectively,which indicating that their ploidy levels were triploid and diploid.In total,240.53 Gb and 87.69 Gb sequences were obtained through HiSeq 2500 sequencing methods,respectively.The genome size of C.esculenta was in the range of 2.08-2.38 Gb.The GC contentsr anged from 42.57%to 42.75%,and the heterozygosity was in the range of 0.52%-2.29%.According to the genomic data of Suyu No.1,the assembled genome sequence was about 2.27 Gb,and contig N50 was 359 bp.A total of 24302 genes were generated,and 24163(about 99.43%)gene sequences were successfully annotated from six public databases.A total of 928987 SSR loci were screened out.
作者
殷剑美
王立
蒋璐
韩晓勇
郭文琦
李春宏
张培通
YIN Jian-mei;WANG Li;JIANG Lu;HAN Xiao-yong;GUO Wen-qi;LI Chun-hong;ZHANG Pei-tong(Institute of Industrial Crops,Jiangsu Academy of Agricultural Sciences,Provincial Key Laboratory of Agrobiology,Nanjing 210014,China)
出处
《江苏农业学报》
CSCD
北大核心
2019年第6期1284-1291,共8页
Jiangsu Journal of Agricultural Sciences
基金
国家自然科学基金项目(31501776)
江苏省农业生物学重点实验室项目(4911707Z201707)
关键词
芋
流式细胞术
基因组测序
基因功能注释
倍型水平
基因组特征
Colocasia esculenta
flow cytometry
genome sequencing
gene function annotation
ploidy level
genomic characterization
