摘要
目的探讨高效液相色谱法(HPLC)在黄精颗粒和提取物的含量测定效果。方法制备黄精颗粒、提取物,采用HPLC法,色谱柱为:Agilent HC-C18(2)色谱柱(4.6 mm×250 mm,5μm),以乙腈(A)和水(B)为流动相,采用梯度洗脱,梯度程序为:0 min:5%A;10 min:10%A;30 min:35%A;40 min:40%A;60 min:5%A。流速1 mL/min,柱温30℃,检测波长200 nm,完成黄精颗粒和提取物含量测定。结果经过与标准品对比可以看书5号峰是薯蓣皂苷元。通过颗粒和原药材色谱图对比得知颗粒和原药材中的化学成分没变化,黄精颗粒中薯蓣皂苷元的含量比原药材中的薯蓣皂苷元含量高。结论将HPLC法用于黄精颗粒和提取物含量测定中效果理想,具有准确、简便,重现性好等优点;黄精颗粒中的薯蓣皂苷元含量比原药材中的薯蓣皂苷元含量高。
Objective To investigate the effect of high performance liquid chromatography(HPLC)on the determination of Huangjing granules and extracts.Methods Preparation of Huangjing granules and extracts by HPLC.The column was Agilent HC-C18(2)column(4.6 mm×250 mm,5μm)with acetonitrile(A)and water(B)as mobile phase.Gradient elution,gradient program:0 min:5%A.10 min:10%A.30 min:35%A.40 min:40%A.60 min:5%A.The flow rate was 1 mL/min,the column temperature was 30°C,and the detection wavelength was 200 nm.The content of Huangjing granules and extract was determined.Results After comparison with the standard product,it can be seen that the peak No.5 was diosgenin.The chromatograms of the granules and the original medicinal materials showed that the chemical constituents in the granules and the original medicinal materials did not change.The content of diosgenin in the granules was higher than that in the original medicinal materials.Conclusion The HPLC method is ideal for the determination of Huangjing granules and extracts.It has the advantages of accuracy,simplicity and reproducibility.The content of diosgenin in Huangjing granules is higher than that in the original medicinal materials.
作者
孙艳平
王荣
杨宽
申旭霁
汪兴军
Sun Yanping;Wang Rong;Yang Kuan;Shen Xuji;Wang Xingjun(Pharmaceutical College of Xian Medical College,Xian 710021,Shanxi,China)
出处
《贵州医药》
CAS
2019年第12期1871-1874,共4页
Guizhou Medical Journal
基金
陕西省教育厅2017年专项科学研究计划(17JK0651)
陕西省中医管理局中医药科研课题(JCMS018)
西安医学院药学省级重点学科建设项目(2016YXXK06)
关键词
高效液相色谱法
黄精颗粒
提取物
测定效果
High performance liquid chromatography
Huangjing granule
Extract
determination effect
