摘要
目的基于LC-MS分析与网络药理学评估多花黄精缓解炎性疲劳(Inflammatory fatigue)的有效成分与活性机制。方法通过LC-MS获得多花黄精成分信息,收集成分作用靶点信息;对比炎性疲劳的基因靶点后获取共有靶点作为多花黄精作用靶点;进行共有基因靶点GO功能富集分析及KEGG通路富集分析,最后采用促炎细胞模型验证多花黄精抗炎活性。结果LC-MS共检测到62个多花黄精化学成分,发现成分与疾病的共有靶点208个。网络药理学分析提示:N-顺阿魏酰酪胺、β-谷甾醇、11 E-十八碳二烯酸、β-D-葡萄糖醛酸和甘草素为多花黄精抗炎性疲劳重要起效成分,STAT3、MAPK3、AKT1、JUN、TP53、EGFR、CASP3、IL6可能为其关键作用靶点,而5-羟色胺能突触、钙信号通路、JAK-STAT信号和NF-κB通路是其主要的生物途径。细胞实验研究表明多花黄精具有显著的抑制LPS诱导的巨噬细胞分泌炎症因子作用,表明多花黄精具有一定的抗炎活性。结论多花黄精中较多的小分子成分可通过多种生物途径,作用于多个生物靶点改善机体炎性相关疲劳。
OBJECTIVE To evaluate the effective components and active mechanism of Polygonatum cyrtonema Hua in relieving inflammatory fatigue based on LC-MS analysis and network pharmacology.METHODS The component information of Polygonatum cyrtonema Hua was obtained through LC-MS,and the information on the targets of the components was collected.After comparing the gene targets of inflammatory fatigue,the common targets were obtained as the targets of Polygonatum cyrtonema Hua;GO functional enrichment analysis and KEGG pathway enrichment analysis of the shared gene targets were performed;finally,a pro-inflammatory cell model was used to verify the anti-inflammatory activity of Polygonatum cyrtonema Hua.RESULTS A total of 62 chemical components of Polygonatum cyrtonema Hua were detected by LC-MS,and 208 common targets between components and diseases were found.Network pharmacological analysis suggested that N-cis-feruloyl tyramide,β-sitosterol,11 E-octadecadienoic acid,β-D-glucuronic acid and liquiritigenin are important active components of Polygonatum cyrtonema Hua against inflammatory fatigue,and STAT3,MAPK3,AKT1,JUN,TP53,EGFR,CASP3 and IL-6 may be the key biological targets.The main biological pathways are focused on 5-HT aminergic synapses,calcium signaling pathway,JAK-STAT signaling and NF-κB pathway.The cell experiments showed that Polygonatum cyrtonema Hua had a significant inhibitory effect on LPS-induced macrophage secretion of inflammatory cytokines,indicating that Polygonatum cyrtonema Hua can exert certain anti-inflammatory actions.CONCLUSION The relatively large number of small molecule components in Polygonatum cyrtonema Hua can act on multiple biological targets through various biological pathways to improve inflammation-related fatigue in the body.
作者
刘涛
嵇晶
王令充
程建明
LIU Tao;JI Jing;WANG Ling-chong;CHENG Jian-ming(School of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China;Jiangsu Province Engineering Research Center of Classical Prescription,Nanjing 210023,China)
出处
《南京中医药大学学报》
CAS
CSCD
北大核心
2023年第9期879-887,共9页
Journal of Nanjing University of Traditional Chinese Medicine
基金
南京中医药大学校企合作项目(2021112)。
