摘要
基于硼酸配基与顺式二羟基化合物的可逆共价结合,硼亲和分子印迹技术具有印迹效率高、亲和力强以及pH调控的结合与释放等优势,被广泛应用于含有顺式二醇配基分子的分离与富集。文章介绍了硼亲和分子印迹技术在核苷酸、糖、糖蛋白、细胞和细菌识别及富集中的应用,并对其发展前景进行了展望。
Boron affinity molecular imprinting technology has the advantages of high imprinting efficiency,strong affinity,and the combination and release of pH regulation because of the reversible covalent binding of boric acid ligands to cis-dihydroxy compounds,and it is widely used in separation and enrichment of cis-diol complexes base molecule.We introduced the application of boron affinity molecular imprinting technology in the identification and enrichment of nucleotides,sugars,glycoproteins,cells and bacteria,and prospected for the future development of this technology.
作者
徐斐
郭猛
叶泰
袁敏
曹慧
于劲松
袁然
徐晓喆
史景灏
苑红恩
XU Fei;GUO Meng;YE Tai;YUAN Min;CAO Hui;YU Jing-song;YUAN Ran;XU Xiao-zhe;SHI Jing-hao;YUAN Hong-en(School of Medical Instrument and Food Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China)
出处
《食品与机械》
北大核心
2019年第12期8-13,共6页
Food and Machinery
基金
国家自然科学基金（编号：31671934）
上海市科委科技支撑（农业重点科技攻关）项目（编号：17391901500）
关键词
分子印迹
硼亲和
顺式二醇生物分子
分离富集
molecular imprinting
boron affinity
cisdiol biomolecule
separation and enrichment
