摘要
生活污水处理系统是抗生素抗性基因(ARGs)重要的“汇”和“源”。为探究ARGs在生活污水处理系统包括污水、污泥处理单元全流程的分布变化和去除效率,选取了河北省某A^2/O生活污水处理系统,使用普通PCR技术、实时荧光定量PCR技术对污水、污泥处理工艺沿程各单元的胞内、胞外ARGs的丰度变化及去除效果进行研究。在污水处理系统中对tetC、sulII、ermB、blaPSE-14种ARGs、Ⅰ型整合子IntI 1以及16S rDNA进行了检测。在总进水中,总胞内ARGs和胞外ARGs绝对丰度分别为1.30×10^8 copies/mL和2.09×10^2 copies/mL,总胞内ARGs和胞外ARGs相对丰度分别为3.16×10^-2和5.29×10^-1。最终出水的总胞内ARGs和胞外ARGs绝对丰度去除log值分别达到2.10log和-3.20log,总胞内ARGs和胞外ARGs相对丰度去除log值分别达到-0.21log和0.60log。泥区废液总回流液的回流会增大总进水胞外ARGs负荷。相关性研究表明,Ⅰ型整合子IntI 1可能会促进tetC、ermB、blaPSE-1的传播,水质因子可能会影响ARGs的分布与扩散。
Domestic wastewater treatment plants(WWTPs)are important“sink”and“source”of antibiotic resistance genes(ARGs).In order to explore the distribution change and removal efficiency of ARGs in the whole process of domestic wastewater treatment plants including wastewater and sludge treatment units,a typical domestic wastewater treatment plant with A^2/O as the main process in Hebei,China was selected in this study.ARGs and other genes in all units of wastewater and sludge treatment process were studied by using common PCR technology and real-time fluorescence quantitative PCR technology for the distribution change and removal effect.Four kinds of ARGs(tetC,sulII,ermB,blaPSE-1),class I integron IntI 1 and 16S rDNA were detected in the wastewater treatment plant.In total influent water,the absolute abundances of total intracellular ARGs and extracellular ARGs were 1.30×10^8 copies/mL and 2.09×10^2 copies/mL,respectively.The relative abundances of total intracellular ARGs and extracellular ARGs were 3.16×10^-2 and 5.29×10^-1,respectively.The absolute abundance removal logs of total intracellular ARGs and extracellular ARGs were 2.10log and-3.20log,respectively.The relative abundance removal logs of total intracellular ARGs and extracellular ARGs were-0.21log and 0.60log,respectively.The total reflux liquid of sludge treatment process to the influent pump station would increase the total inflow extracellular ARGs load.Correlation analysis showed that classⅠintegron IntI 1 might promote the transmission of tetC,ermB and blaPSE-1,and water quality might affect the distribution and diffusion of ARGs.
作者
谢辉
包樱钰
李菲菲
温东辉
陈吕军
XIE Hui;BAO Ying-yu;LI Fei-fei;WEN Dong-hui;CHEN Lv-jun(School of Environment,Tsinghua University,Beijing 100084,China;College of Environmental Sciences and Engineering,Peking University,Beijing 100871,China)
出处
《环境工程》
CAS
CSCD
北大核心
2019年第12期80-89,共10页
Environmental Engineering
基金
国家自然科学基金项目(51678003,51678334)
