摘要
Pokemon是一种转录抑制因子,能够通过影响染色质的重组或直接与抑癌基因结合而抑制抑癌基因的转录,促使肿瘤形成。该文利用基于液相色谱-质谱联用的代谢组学技术研究了Pokemon在肝癌中调控细胞代谢的作用机制。通过脂质转染,获得了Pokemon高表达的HL7702细胞,分别收集转染后不同时间点的细胞。利用基于液相色谱-质谱联用技术的代谢组学方法,分析胞内代谢物的成分。根据多元统计分析的结果选出差异显著的候选代谢物,通过数据库(METLIN和HMDB)检索、二级图谱比对进行结构解析,确证了36种代谢物。通过KEGG数据库检索发现这些代谢物主要与脂质合成相关。进一步分析发现脂质合成途径中乙酰辅酶羧化酶和脂肪酸合成酶均被激活。结果显示,Pokemon可通过激活细胞中脂质合成通路而影响细胞的代谢。
Pokemon,a transcriptional repressor,inhibits the transcription of tumor suppressor genes and promotes tumor formation by affecting chromatin recombination or binding directly to the tumor suppressor genes.In this study,the mechanism behind the Pokemon regulation of liver cancer cell metabolism was evaluated using liquid chromatography-mass spectrometry(LC-MS)-based metabolomics.Pokemon-overexpressing HL7702 cells were obtained by lipofection.The cells were collected at different time points after transfection and their intracellular metabolites were analyzed using an LC-MS-based metabolomic method.Based on the results of a multivariate statistical analysis,candidate metabolites with significant differences were selected,and 36 of them were confirmed by database and MS/MS analysis.These metabolites were primarily involved in lipid synthesis by KEGG database searches.Further analysis indicated that both the acetyl-coenzyme carboxylase and the fatty acid synthase were activated in the lipid synthesis pathway.The results show that Pokemon can affect cell metabolism by activating the lipid synthesis pathways in cells.
作者
金一宝
李上富
王珏
高丹
刘红霞
蒋宇扬
殷果
王铁杰
JIN Yibao;LI Shangfu;WANG Jue;GAO Dan;LIU Hongxia;JIANG Yuyang;YIN Guo;WANG Tiejie(Shenzhen Institute for Drug Control,Shenzhen Key Laboratory of Drug Quality Standard Research,Shenzhen 518057,China;Graduate School at Shenzhen,Tsinghua University,Shenzhen 518055,China)
出处
《色谱》
CAS
CSCD
北大核心
2020年第2期162-168,共7页
Chinese Journal of Chromatography
基金
中国博士后基金(2015M572370)
深圳市基础研究项目(JCYJ2016053017352098)~~
