摘要
以麦积山野生刺五加为原料,研究了其有效成分总多酚和总黄酮的超声波辅助提取工艺及体外抗氧化活性。通过单因素试验和正交试验,确定最佳工艺条件为:超声时间40 min、超声功率600 W、提取温度60°C、料液比1∶35(g∶mL),并对此条件进行三次平行验证实验,得出平均提取量为总多酚25.21±0.17 mg GAE/g和总黄酮28.11±0.19 mg RE/g;以人工合成V C为对照,进行了体外抗氧化活性的研究,得出提取物质量浓度达到300mg/L时,总抗氧化能力最大;提取物质量浓度达到440.00 mg/L时,DPPH自由基清除能力最强,清除率为94.52%±1.30%;提取物质量浓度达到130.00 mg/L时,超氧阴离子自由基(O-·2)清除能力最强,清除率为86.71%±1.28%;提取物质量浓度达到100.00 mg/L时,羟基自由基(·OH)清除能力最强,清除率为94.23%±1.53%。整体来看,野生刺五加提取物体外抗氧化能力显著强于人工合成的V C(P<0.05)。
Using Maijishan wild Acanthopanax as raw material,the parameters of ultrasonic assisted extraction process and the antioxidant activity in vitro of total polyphenols and flavonoids were studied.The better technological conditions as follows∶ultrasonic time 40 min,ultrasonic power 600 W,ultrasonic temperature 60°C,solid-liquid ratio 1∶35(g∶mL).The process parameters were determined by using single factor test,orthogonal test,and three parallel validation experiments.The average extraction amount of total polyphenols is 25.21±0.17 mg GAE/g and total flavonoids is 28.11±0.19 mg RE/g.Using synthetic Vc as the control,the antioxidant activity in vitro was studied.The results showed that the total antioxidant capacity was the largest when the extract concentration reached 300 mg/L.When the concentration of extract reached 440.00 mg/L,DPPH free radical scavenging ability was the strongest,and the scavenging rate was 94.52%±1.30%.When the concentration of extract reached 130.00 mg/L,superoxide anion radical(O-·2)had the strongest scavenging ability,and the scavenging rate was 86.71%±1.28%.When the concentration of extract reached 100.00mg/L,the scavenging ability of hydroxyl radical(·OH)was the strongest,and the scavenging rate was 94.23%±1.53%.Overall,the antioxidant capacity of wild acanthopanax extract in vitro was significantly stronger than that of synthetic Vc(P<0.05).
作者
王彦博
石燕
袁毅君
WANG Yan-bo;SHI Yan;YUAN Yi-jun(School of Chemical Engineering and Technology,Tianshui Normal University,Tianshui 741000,China)
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2019年第12期2153-2162,共10页
Natural Product Research and Development
基金
国家自然科学基金(81360619)
关键词
刺五加
多酚与黄酮
提取
抗氧化活性
acanthopanax
polyphenols and flavonoids
extraction
antioxidant activity
