摘要
目的:应用组织工程原理培养含黑素细胞的自体组织工程表皮,研究移植治疗白癜风的临床效果。方法:选择稳定期白癜风患者32例(共计白癜风皮损109块),于腹股沟区手术取皮获取自体皮肤细胞,应用组织工程方法培养含黑素细胞的自体组织工程表皮,移植治疗稳定期白癜风,随访观察3个月;应用皮肤计算机数字图像等方法评价移植治疗后的临床效果。结果:对109块白癜风皮损进行了含黑素细胞的自体组织工程表皮移植,移植治疗后总有效率为85.32%;计算机图像分析系统显示移植后的平均面积为(194.97±2.33)cm 2,逐渐接近移植前的(226.32±2.34)cm 2;移植后复色的平均灰度为(109.87±2.02),逐渐接近移植前的(113.13±2.36);随访过程中未见明显的不良反应。结论:应用含黑素细胞的自体组织工程表皮移植治疗白癜风,移植后复色效果好,移植面积明显增大,且无明显并发症,值得临床推广应用。
Objective:To culture the autologous tissue engineered epidermis included melanocytes by tissue engineering technique,and to research the clinical effect after the grafting for treatment stable vitiligo.Methods:Thirty-two patients with vitiligo were selected in this study,a total of 109 vitiligo lesions.The skin cells were obtained from operation on grion and then cultured the autologous tissue engineered epidermis included melanocytes.The vitiliginous lesion was grafted for treatment of vitiligo,and the patients were followed up for 3 months.The clinical effect was evaluated by computer digital image of skin.Results:The total 109 chunks of body vitiligo lesion to be grafted by autologous epidermis,the overall response rate was 85.32%.The computer digital image analysis system showed the average area after grafting was(194.97±2.33)cm 2,which was near before grafting(226.32±2.34)cm 2,average gray before and after grafting was namely(109.87±2.02)and(113.13±2.36),and the data was also near close each other.No adverse reactions were observed during follow-up.Conclusion:Autologous tissue engineered epidermis included melanocytes cultured and grafted is relatively excellent effect,the grafted treatment area is relative increment.There are no side effect appeared in clinical,which is valuable in clinical application.
作者
罗卫
陈杰
徐劲
徐印祥
LUO Wei;CHEN Jie;XU Jin;XU Yinxiang(Department of Dermatology,Air Force Special Medical Center of PLA,Beijing 100142,China;Beijing Emmett Biotechnology Corporation Limited Company)
出处
《西北国防医学杂志》
CAS
2019年第12期737-741,共5页
Medical Journal of National Defending Forces in Northwest China
基金
首都临床特色应用研究科研课题(Z141107002514170)
关键词
白癜风
组织工程表皮
移植疗效
计算机图像
vitiligo
tissue engineering epidermis
grafting effect
computer image
