摘要
以菘蓝幼苗为研究对象,探究H 2S缓解菘蓝幼苗镉(Cd)胁迫的最佳浓度及作用机制.在Cd浓度为5 mg·L-1条件下,确定缓解Cd胁迫的H 2S最佳浓度,并在最佳浓度下,设置不同镉Cd(0,1.0,2.5,5)mg·L-1胁迫浓度,探究H 2S在缓解菘蓝幼苗Cd胁迫中的作用.结果显示,外源添加H 2S供体NaHS后,不同浓度Cd胁迫下菘蓝幼苗与处理组相比,株高分别增加8.47%、12.49%、5.08%;在低浓度Cd处理时加入NaHS,叶绿素含量显著性增加47.10%、28.12%,而5.0 mg·L-1 Cd胁迫下叶绿素含量显著降低了25.85%.此外,外源添加NaHS,地下部分Cd含量分别增加34.40%、34.60%、41.76%,地上部分Cd含量分别减少52.84%、46.69%、43.23%.综上所述,外源H 2S能够通过调节菘蓝幼苗体内Cd的分布降低Cd对菘蓝幼苗造成的损伤.而高浓度Cd会引起菘蓝幼苗生长异常,导致幼苗对H 2S更加敏感,此时H 2S可能表现出毒性作用而非缓解Cd胁迫的作用.
In this study,Isatis indigotica seedlings were used as research objects to explore the optimal concentration of H 2S to alleviate cadmium(Cd)stress in Isatis indigotica seedlings and the mechanism.Under the condition of Cd concentration of 5 mg·L-1,the optimal concentration of H 2S for Cd stress was determined,and different concentrations of Cd were set at the optimal concentration to exploring the role of H 2S in alleviating Cd stress in Isatis indigotica seedlings.The results showed that the plant height of the Isatis indigotica seedlings increased by 8.47%,12.49%,and 5.08%,respectively,after the addition of H 2S donor NaHS.When NaHS was added at low concentration of Cd,the chlorophyll content increased significantly by 47.10%and 28.12%,while chlorophyll content decreased by 25.85%under 5 mg·L-1 Cd.In addition,the addition of NaHS to the external sources increased the Cd content in the underground part by 34.40%,34.60%,and 41.76%,respectively,and the Cd content in the aboveground part decreased by 52.84%,46.69%,and 43.23%,respectively.In summary,exogenous H 2S can reduce the damage of Cd to Isatis indigotica seedlings by regulating the distribution of Cd in Isatis indigotica seedlings.Because the high concentration of Cd causes abnormal growth of seedlings,seedlings are more sensitive to H 2S.Therefore,H 2S may exhibit toxic effects instead of alleviating Cd stress.
作者
贾红磊
刘华欣
马佩云
王肖
JIA Hong-lei;LIU Hua-xin;MA Pei-yun;WANG Xiao(School of Environmental Science and Engineering,Shaanxi University of Science&Technology,Xi′an 710021,China)
出处
《陕西科技大学学报》
CAS
2020年第1期32-37,共6页
Journal of Shaanxi University of Science & Technology
基金
国家自然科学基金项目(31700445)
关键词
硫化氢
菘蓝
镉胁迫
叶绿素
hydrogen sulfide
Isatis indigotica
cadmium stress
chlorophyll
