摘要
为研究纳米银(Ag NPs)与镉(Cd^(2+))对拟南芥的复合毒性,本文以不同浓度的PVP coated-Ag NPs和Cd^(2+)处理拟南芥幼苗一周后,测定了拟南芥幼苗主根长度,根毛数量,叶片中光合色素和可溶性蛋白含量及抗氧化系统相关酶活性.结果表明,高浓度(5mg/L)Cd^(2+)能显著抑制根长,提高可溶性蛋白含量及SOD(超氧化物歧化酶)和POD(过氧化物酶)的活性.在高浓度Cd^(2+)(5mg/L)中加入Ag NPs能显著增强Cd^(2+)对根长的抑制作用,而高浓度的Cd^(2+)能缓解Ag NPs对根毛的抑制作用;在低浓度Cd^(2+)(0.1mg/L和1mg/L)中加入Ag NPs能显著增强Cd^(2+)对色素含量抑制作用;在含有Cd^(2+)的处理中,SOD和POD在1mg/L Ag NPs下活性最高;以上结果说明Cd^(2+)和Ag NPs对拟南芥幼苗根系形态及叶片生理指标都存在交互影响.
To investigate the combined toxictiy of Ag NPs and Cd^（2＋）on Arabidopsis thaliana seedlings,the root length,the number of root hairs,the content of photosynthetic pigment and protein,superoxide dismutade（SOD）and peroxidase（POD）activity were measured after one week exposure to different concentrations of PVP coated-Ag NPs and Cd^（2＋）.Results showed that high concentration（5mg/L）of Cd^（2＋）could significantly inhibit root elongation and increase the protein content and SOD,POD activity.After adding Ag NPs in high concentration（5mg/L）of Cd^（2＋）treatment,the inhibition effect of Cd^（2＋）on Arabidopsis thaliana root length was significantly increased while the inhibition effect of Ag NPs on Arabidopsis thaliana root hairs was decreased.After adding Ag NPs in low concentration（0.1and 1mg/L）of Cd^（2＋）treatment,the inhibition effect of Cd^（2＋）on Arabidopsis thaliana photosynthetic pigment content were significantly increased.In Cd^（2＋）treatment,SOD,POD activity reached the highest when the seedlings were exposured to 1mg/L Ag NPs.Above results showed that Cd^（2＋）and Ag NPs had interaction effects on root morphology and leaves physiological indexes of Arabidopsis thaliana.
作者
张传玲
江红生
李长江
汤棋惠
王悠
尹黎燕
ZHANG Chuan-ling1, JIANG Hong-sheng2, LI Chang-jiang1, TANG Qi-hui1, WANG You1, YIN Li-yan1.(1 .Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China; 2.Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China)
出处
《中国环境科学》
EI
CAS
CSSCI
CSCD
北大核心
2018年第5期1951-1960,共10页
China Environmental Science
基金
国家自然科学基金资助项目(31460089,31170481)
海南大学科研启动基金资助项目(kyqd(zr)1707)
关键词
纳米银
镉
复合毒性
silver nanoparticle
cadmium
combined toxicity
