摘要
目的采用RP-HPLC-UV色谱法同时测定清心莲子饮中人参皂苷Re、Rg1、Rb1的含量影响。方法采用色谱柱:依利特C18柱(Sinochrom ODS-BP,4.6mm×200mm,5μm),流动相采用A为乙腈,B为水,梯度洗脱,流速为1.0mL·min^-1,进样量为10μL,检测波长为203nm,柱温25℃,理论塔板数大于6000。结果在100min内,人参皂苷Re、Rg1、Rb1分离度良好,进样的含量与色谱峰面积具有良好的线性关系,人参皂苷Re、Rg1、Rb1的线性方程分别为:y=0.5752x-0.0961(r=0.9994)、y=1.3157x-0.2006(r=0.9998)、y=0.6637x+0.2091(r=0.9997),人参皂苷Re、Rg1、Rb1的平均加样回收率分别是:101.08%(RSD=1.77%)、99.40%(RSD=2.55%)、100.11%(RSD=2.83%)。结论本文建立的含量测定方法快捷稳定可靠,加样回收率高,适用于清心莲子饮中人参皂苷Re、Rg1、Rb1的含量测定,为清心莲子饮的质量评价提供了理论支持和实验依据。
OBJECTIVE RP-HPLC-UV method was used to determine the contents of ginsenoside Re,Rg1 and Rb1 in Qingxin Lianzi.METHODS The analysis was performed on an Sinochrom ODS-BP(4.6mm×200mm,5μm)reverse phase column.The flow rate was 1.0mL·min^-1,the injection volume was 10μL.The mobile phase consisted of water and acetonitrilewas used for gradient elution.The detection wavelength was 203 nm and the column temperature was 25℃.RESULTS The ginsenosides Re,Rg1 and Rb1 were completely separated in the Qingxin Lianzi within 100min.The linearity of the ginsenosides Re,Rg1 and Rb1 was linear with the mass of the Qingxin Lianzi and the peak area of the chromatogram.The linear equations of ginsenosides Re,Rg1 and Rb1 were y=0.5752x-0.0961(r=0.9994),y=1.3157x-0.2006(r=0.9998),y=0.6637x+0.2091(r=0.9997),respectively.The average recoveries of ginsenosides Re,Rg1 and Rb1 were 101.08%(RSD=1.77%),99.40%(RSD=2.55%),100.11%(RSD=2.83%).CONCLUSION The content determination method established in this paper is fast,stable and reliable with high recovery rate.It is suitable for the determination of ginsenoside Rg1,Rb1 and Re in.It provides theoretical support and experimental basis for the quality evaluation of Qingxin Lianzi.
作者
苟鑫宇
季宏与
刘玉婷
逄弓一郎
卓小玉
田明
GOU Xin-yu;JI Hong-yu;LIU Yu-ting;PANG Gong-yi-lang;ZHUO Xiao-yu;TIAN Ming(Key Laboratory of Chinese Materia Medica(Ministry of Education)Laboratory of Pharmacy on Chinese Materia Medica,Heilongjiang University of Chinese Medicine,Harbin 150040,China)
出处
《化学工程师》
CAS
2019年第12期21-24,共4页
Chemical Engineer
基金
黑龙江中医药大学“中药经典名方清心莲子饮制剂研发”科研项目(404H076)
