摘要
捕捉细胞内分子活动发生的动态过程,从细胞分裂到囊泡运输再到胞内钙离子浓度变化等大量快速发生和发展的生理过程是很多科研工作者的需要。这个过程不仅要求较高的时间分辨率,还要求较低的激发光强度以使样品的淬灭和光损伤达到最小。因此,相机的高灵敏度图像传感器起到了决定作用。鉴于近几年图像传感器发展迅速,本文对其进行了系统的阐述,综述了相机的图像传感器技术上的最新突破和改进。进而,总结了其在活细胞成像中的应用,对比了两种主流传感器并展望了未来发展趋势。
To capture the dynamic process of intracellular molecular activity,a large number of rapidly occurring and developing physiological processes from cell division to vesicle transport to intracellular calcium ion concentration changes are widely needed in relevant researches.This process requires not only higher time resolution,but also lower excitation light intensity to minimize quenching and photodamage effects to samples.Therefore,the camera’s high-sensitivity image sensor plays a decisive role.In light of the rapid development of image sensors in recent years,this paper systematically reviews the latest developments in camera image sensor technology.Furthermore,its application in live cell imaging is summarized,two widely adopted sensors are compared and the future development prospects are also discussed.
作者
刘丽娜
薛冰
魏华
李华
薛奋勤
许晴
LIU Lina;XUE Bing;WEI Hua;LI Hua;XUE Fenqin;XU Qing(Core Facilities Centre,Capital Medical University,Beijing 100069,China)
出处
《激光生物学报》
CAS
2019年第6期513-517,共5页
Acta Laser Biology Sinica
基金
首都医科大学校长基金项目(2016JS13)
关键词
图像传感器
活细胞成像
电子倍增电子耦合元件
科学互补金属氧化物半导体
image detectors
live cell imaging
electron-multiplying charged-coupled device(EMCCD)
scientific complementary metal-oxide semiconductor(sCMOS)
