摘要
目的:通过测定抑郁模型大鼠脑内氨基酸类神经递质Glu及GABA水平的变化,探讨归脾汤抗抑郁的作用机制。方法:选用Wistar大鼠60只,雌、雄各半。随机分组,10只为正常组,其余50只造模后分为模型组、百忧解组、归脾汤高、中、低剂量组。实验中记录体质量变化,进行行为学评分,检测脑内Glu及GABA水平。结果:模型组体质量减轻,行为学得分降低,脑内Glu水平升高,GABA水平降低。归脾汤各组大鼠体质量及行为学得分提高,Glu水平降低,GABA水平差异无统计学意义。结论:归脾汤能降低抑郁大鼠脑内Glu水平,对GABA无明显影响,可能通过降低Glu水平而发挥抗抑郁作用。
Objective:To investigate the anti-depression mechanism of Guipi decoction by measuring the changes of amino acid neurotransmitters Glu and GABA in the brain of model rats with depression.Methods:60 rats with equal amount of male and female were chosen for this experiment.10 rats were kept in normal group,while the other 50 were randomly divided into five groups:model group,control group,Guipi decoction high dose group,Guipi decoction medium dose group and Guipi decoction low dose group.Changes in body weight were recorded,behavioral scoring were conducted,and Glu and GABA levels in the rat brain were tested.Results:The body weight of the rats in model group decreased,their behavioral score reduced,the Glu level in the brain increased while the GABA level decreased.The body weight and behavioral scores of the rats in Guipi decoction increased,the Glu level decreased,and there was no significant difference on GABA level.Conclusion:Guipi decoction can reduce the level of Glu in the brain of depressed rats,and has no obvious effect on GABA,this may result in antidepressant effect by lowering Glu level.
作者
陈宝忠
潘彦辰
李志强
张璐
Chen Baozhong;Pan Yanchen;Li Zhiqiang;Zhang Lu(Heilongjiang University of Chinese Medicine,Harbin,150040,China)
出处
《成都中医药大学学报》
2019年第4期41-44,共4页
Journal of Chengdu University of Traditional Chinese Medicine
基金
段富津国医大师传承工作室建设项目(国中医药办人教发[1015]24号)
黑龙江省中医药科技计划项目(ZHY16-001)
